{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:07:24Z","timestamp":1785514044129,"version":"3.56.0"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T00:00:00Z","timestamp":1622678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/22728\u20102"],"award-info":[{"award-number":["2019\/22728\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/11680\u20109"],"award-info":[{"award-number":["2019\/11680\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/11450\u20103"],"award-info":[{"award-number":["2019\/11450\u20103"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ozone (O<jats:sub>3<\/jats:sub>) therapy has been used to improve peripheral tissue oxygenation in humans and domestic animals. The goal of the present study was to characterize histological changes in the endometria of healthy equines following tissue exposure to gas mixtures enriched with different concentrations of O<jats:sub>3<\/jats:sub>. Cycling mares without endometrial degeneration were divided into three groups according to treatment (<jats:italic>n<\/jats:italic>\u00a0=\u00a09\u00a0mares\/group). The uteri from the O<jats:sub>3<\/jats:sub>, \u00bdO<jats:sub>3<\/jats:sub> and control groups were insufflated for 3\u00a0min with gas containing 42, 21 and 0\u00a0\u03bcg O<jats:sub>3<\/jats:sub> ml<jats:sup>\u20101<\/jats:sup>, respectively. Treatments were performed every three days from D0 to D6. Endometrial samples were collected immediately before the first treatment and 24\u00a0hr after the last treatment. The following nine histological parameters were evaluated: (i) the number of endometrial blood vessels, (ii) endometrial vascular degree (EVD), (iii) increase rate of blood vessels, (iv) increase rate of EVD, (v) glandular total area, (vi) glandular lumen area, (vii) intraglandular secretion area, (viii) glandular epithelial height and (ix) luminal epithelial height. In the O<jats:sub>3<\/jats:sub> group, a positive effect from treatment (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) was detected for all vascular parameters (i, ii, iii and iv), glandular total area, intraglandular secretion area and glandular epithelial height. Compared to the control group, the \u00bdO<jats:sub>3<\/jats:sub> group had greater (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) EVD (84.1\u00a0\u00b1\u00a012%) and a higher increase rate of blood vessels (151.9\u00a0\u00b1\u00a047.1%). Uterine insufflation with low or intermediate concentrations of the O<jats:sub>2<\/jats:sub>\u2013O<jats:sub>3<\/jats:sub> gas mixture induced endometrial angiogenesis. Morphometry, but not morphology, of the endometrial glands was affected by local O<jats:sub>3<\/jats:sub> therapy. These findings would be of great significance for the development of new therapies for infertility in mares.<\/jats:p>","DOI":"10.1111\/rda.13958","type":"journal-article","created":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T13:38:47Z","timestamp":1621690727000},"page":"1176-1183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Endometrial angiogenesis induced by uterine insufflation with an oxygen\u2013ozone gas mixture in mares"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6908-3463","authenticated-orcid":false,"given":"Jair","family":"Camargo Ferreira","sequence":"first","affiliation":[{"name":"Post\u2010graduation Program in Animal Science University of Franca Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Silva Amaral","sequence":"additional","affiliation":[{"name":"Post\u2010graduation Program in Animal Science University of Franca Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia","family":"Mara Cardoso","sequence":"additional","affiliation":[{"name":"Post\u2010graduation Program in Animal Science University of Franca Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Panelli Marchi\u00f3","sequence":"additional","affiliation":[{"name":"Post\u2010graduation Program in Animal Science University of Franca Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Aldrovani Rodrigues","sequence":"additional","affiliation":[{"name":"Post\u2010graduation Program in Animal Science University of Franca Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo","family":"Fonseca Alves","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinics School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cezinande","family":"de Meira","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2015.08.880"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2005.11.005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2004.00885.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64066-7"},{"key":"e_1_2_11_6_1","first-page":"270","article-title":"Biological and clinical effects of ozone. Has ozone therapy a future in medicine?","volume":"56","author":"Bocci V. A.","year":"1999","journal-title":"British Journal of Biomedical Science"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2005.08.006"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2012.693609"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00084-0"},{"key":"e_1_2_11_10_1","first-page":"149","article-title":"A novel terapeutic option for Chronic Fatigue Syndrome and Fibromyalgia","volume":"1","author":"Borrelli E.","year":"2002","journal-title":"Rivista Italiana Di Ossigeno\u2010Ozonoterapia"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1992.tb02835.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/01443615.2015.1133579"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.09.018"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.975"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0737-0806(84)80064-7"},{"key":"e_1_2_11_16_1","first-page":"721","article-title":"Histological pattern of endometrial samples in postmenopausal women with abnormal uterine bleeding","volume":"28","author":"Deeba F.","year":"2016","journal-title":"Journal of Ayub Medical College Abbottabad"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030634"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12475"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-017-0236-7"},{"key":"e_1_2_11_20_1","first-page":"103","article-title":"Ozone treatment of metritis and endometritis in Holstein cows zone treatment of metritis and endometritis in Holstein cows","volume":"84","author":"\u0110uri\u010di\u0107 D.","year":"2014","journal-title":"Veterinarski Arhiv"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12715"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01805.x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mefs.2015.04.005"},{"key":"e_1_2_11_24_1","first-page":"e20180015","article-title":"Effect of topical ozonetherapy on gingival wound healing in pigs: Histological and immuno\u2010histochemical analysis","volume":"27","author":"Eroglu Z. T.","year":"2019","journal-title":"Journal of Applied Oral Science"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-020-02298-3"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.04.014"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.06.013"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0447"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.07.008"},{"key":"e_1_2_11_30_1","first-page":"89","volume-title":"Ultrasonic imaging and animal reproduction: Horses","author":"Ginther O. J.","year":"1995"},{"key":"e_1_2_11_31_1","first-page":"23","volume-title":"Ultrasonic imaging and animal reproduction: Horses","author":"Ginther O. J.","year":"1995"},{"key":"e_1_2_11_32_1","volume-title":"Ultrasonic imaging and animal reproduction: Color\u2010doppler ultrasonography","author":"Ginther O. J.","year":"2007"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(98)80051-0"},{"key":"e_1_2_11_34_1","first-page":"427","article-title":"Studies on experimental growth retardation in sheep. The effects of a small placenta in restricting transport to and growth of the fetus","volume":"7","author":"Harding J. E.","year":"1985","journal-title":"Journal of Developmental Physiology"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1983.tb01826.x"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0803894"},{"key":"e_1_2_11_37_1","first-page":"723","article-title":"Equine endometrial biopsy","author":"Kenney R. M.","year":"1986","journal-title":"Current Therapy in Theriogenology"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASW.0000484172.04260.46"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.06.001"},{"key":"e_1_2_11_40_1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.4103\/2045-9912.260652","article-title":"Ozone therapy: A potential therapeutic adjunct for improving female reproductive health","volume":"9","author":"Merhi Z.","year":"2019","journal-title":"Medical Gas Research"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2007.038588"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mefs.2018.04.006"},{"key":"e_1_2_11_43_1","first-page":"860","article-title":"The Effect of Ozone Therapy on Experimental Vasospasm in the Rat Femoral Artery","volume":"26","author":"Orakdogen M.","year":"2016","journal-title":"Turkish Neurosurgery"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2011.00446.x"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.5935\/0004-2749.20140054"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-865020160070000007"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.11.025"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.036384"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2007.06.010"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880302600404"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2010.00649.x"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.22525.1"},{"key":"e_1_2_11_53_1","first-page":"1","article-title":"Intrauterine ozone administration for improving fertility rate in ntrauterine ozone administration for improving fertility rate in Simmental cattle","volume":"84","author":"Zobel R.","year":"2014","journal-title":"Veterinarski Arhiv"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13958","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13958","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13958","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T17:37:56Z","timestamp":1692985076000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13958"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,3]]},"references-count":52,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/rda.13958"],"URL":"https:\/\/doi.org\/10.1111\/rda.13958","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,3]]},"assertion":[{"value":"2021-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}