{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:58:34Z","timestamp":1781967514902,"version":"3.54.5"},"reference-count":8,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:00:00Z","timestamp":1625184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of the study was to investigate the efficiency of three enrichment methods to separate boar spermatozoa. Twenty\u2010four ejaculates from 12 boars (2 ejaculates\/boar) were extended (30\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> spermatozoa\/mL) in commercial Beltsville Thawing Solution. Each semen sample was processed with glass wool column (GW) and glass beads (GB) filtration and with the single\u2010layer centrifugation (SLC) technique. Semen samples before (control; C) and after treatment were evaluated for sperm CASA motility\/kinetics and concentration, viability, morphology and chromatin integrity. Data were analysed with mixed models. The concentration of total and motile spermatozoa was significantly decreased after treatment in groups GW and SLC, but not in group GB. Group GW showed increased values of WOB compared with both groups C and GB. Group GB showed greater values of rapid movement spermatozoa and lower values of slow movement spermatozoa compared with group C. In group SLC, higher values of VSL, LIN and STR were observed compared with group C. In conclusion, all techniques under examination enhanced various CASA variables. Based on our results, the GB method is a promising alternative separation technique for boar sperm and deserves further research regarding swine in vitro fertilization.<\/jats:p>","DOI":"10.1111\/rda.13976","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T15:32:24Z","timestamp":1624635144000},"page":"1148-1151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Efficiency of three boar sperm enrichment techniques"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-6654","authenticated-orcid":false,"given":"Athina","family":"Basioura","sequence":"first","affiliation":[{"name":"Unit of Biotechnology of Reproduction Farm Animals Clinic School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2913-3651","authenticated-orcid":false,"given":"Georgios","family":"Tsousis","sequence":"additional","affiliation":[{"name":"Unit of Biotechnology of Reproduction Farm Animals Clinic School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantin M.","family":"Boscos","sequence":"additional","affiliation":[{"name":"Unit of Biotechnology of Reproduction Farm Animals Clinic School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1623-515X","authenticated-orcid":false,"given":"Ioannis A.","family":"Tsakmakidis","sequence":"additional","affiliation":[{"name":"Unit of Biotechnology of Reproduction Farm Animals Clinic School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.02.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01221.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2019.01.017"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.02.025"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"193","article-title":"Inhibition of sperm motility does not affect live\u2010dead separation of bull sperm by glass beads","volume":"3","author":"Foote R. 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