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Currently, the regulatory role of miRNA in the epididymis is poorly understood. Here, transcriptome sequencing was used to analyse miRNA expression profiles in three regions of the epididymis of rams, including caput, corpus and cauda. The results showed that there were 13 known miRNAs between the caput and corpus controls, 29 between the caput and cauda and 22 differences between the corpus and cauda. Based on the analysis of miRNA target genes by GO and KEGG, a negative regulation network of miRNA\u2013mRNA was constructed in which let\u20107, miR\u2010541\u20105p, miR\u2010133b and miR\u2010150 may play an important regulatory role in the maturation regulation of ram epididymal sperm. This research provides a reference for studying the regulation mechanism of sperm maturation in male epididymis and improving semen quality and male reproductive performance.<\/jats:p>","DOI":"10.1111\/rda.13978","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T08:50:40Z","timestamp":1624611040000},"page":"1209-1219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Study of microRNA Expression Profile in Different Regions of Ram Epididymis"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5863-5744","authenticated-orcid":false,"given":"Cuiling","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Science Xinjiang Agricultural University Urumqi China"},{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"},{"name":"Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhai","sequence":"additional","affiliation":[{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Branch of Animal Husbandry Jilin Academy of Agricultural Sciences Gongzhuling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kechuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Wool sheep &amp; 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