{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:40:37Z","timestamp":1763037637222,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T00:00:00Z","timestamp":1629331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to describe successful cryopreservation of sperm from maned wolves (<jats:italic>Chrysocyon brachyurus<\/jats:italic>). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio<jats:sup>\u00ae<\/jats:sup> (Botupharma, Botucatu, Brazil) or Tris\u2013yolk egg extender. Spermatozoa were thawed at 37\u00baC\/30s or 70\u00baC\/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility &gt;55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris\u2013yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post\u2010thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs.<\/jats:p>","DOI":"10.1111\/rda.13999","type":"journal-article","created":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T17:19:00Z","timestamp":1627924740000},"page":"1464-1469","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["First successful frozen semen of the maned wolf (<i>Chrysocyon brachyurus<\/i>)"],"prefix":"10.1111","volume":"56","author":[{"given":"Laura O.","family":"Teodoro","sequence":"first","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9802-4569","authenticated-orcid":false,"given":"Laiza S.","family":"Camargo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0726-7934","authenticated-orcid":false,"given":"Ver\u00f4nica F. 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