{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T21:24:15Z","timestamp":1768771455109,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,5]],"date-time":"2021-12-05T00:00:00Z","timestamp":1638662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008418","name":"Guru Angad Dev Veterinary and Animal Sciences University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008418","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Small heat shock protein B1 (HspB1) has been reported to play an essential role in thermotolerance. This study aimed to determine a correlation (if any) between HspB1 expression and age at first egg lay (puberty) in a native cross\u2010layer poultry of Punjab under heat stress. Forty native cross\u2010layer birds were reared in two different seasons, viz. summer (THI was more than 27), classified as the heat\u2010stressed group (<jats:italic>n<\/jats:italic>\u00a0=\u00a020) and winter season (THI was less than 21), classified as the control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a020). Blood was collected from both the groups of birds in their 15<jats:sup>th<\/jats:sup> week of age and at puberty. Serum catalase and superoxide dismutase activities, reduced glutathione and corticosterone concentration and lipid peroxidation were measured to assess the oxidative stress in both the groups of birds. The serum antioxidants significantly decreased whilst corticosterone levels and lipid peroxidation significantly elevated in birds in response to summer heat stress. Moreover, in summer season, the activities of the antioxidant enzymes further decreased and lipid peroxidation further increased significantly in birds from their pre\u2010pubertal stage to puberty, which was not observed during the winter season. A clone of chicken HspB1 in BL21 (DE3) cells was revived, and recombinant HspB1 was purified using Ni\u2010NTA agarose column. Serum HspB1 concentration was estimated in different groups of birds by indirect ELISA that has been standardized using the recombinant chicken HspB1. Compared to the control, birds under heat stress had significantly higher serum HspB1 levels. The delay in puberty of all the heat\u2010stressed birds was significantly associated with the increase in their serum HspB1 levels. Taken together, the expression of HspB1 was found to be associated with age at puberty in the native cross poultry layers of Punjab.<\/jats:p>","DOI":"10.1111\/rda.14058","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T16:39:09Z","timestamp":1638290349000},"page":"284-291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Delay in puberty is dependent on heat shock protein B1 expression in native cross layers of Punjab under heat stress"],"prefix":"10.1111","volume":"57","author":[{"given":"Astha","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Microbial and Environmental Biotechnology College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2641-6859","authenticated-orcid":false,"given":"B. V. Sunil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbial and Environmental Biotechnology College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prem Prakash","family":"Dubey","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding College of Veterinary Sciences Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neeraj","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding College of Veterinary Sciences Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.5402\/2011\/838606"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1997.6917"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(70)90083-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1982.542309x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/af\/vfy031"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00208-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.2.221"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2010-00728"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07827.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00448-9"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36704"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4314\/nvj.v39i2.2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-008-0068-7"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2010.90053"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10113-018-1359-x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani3020356"},{"key":"e_1_2_9_19_1","first-page":"380","article-title":"Plasma SH and GSH measurement","volume":"233","author":"Lin Hu M.","year":"1988","journal-title":"Methods in Enzymology"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_9_21_1","first-page":"184","article-title":"Microtiter plate assay for SOD using MTT reduction by superoxide","volume":"35","author":"Madesh M.","year":"1998","journal-title":"Indian Journal of Biochemistry & Biophysics"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13593"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408440601123552"},{"issue":"7","key":"e_1_2_9_24_1","first-page":"107","article-title":"Effect of long\u2010term heat stress on key enzyme activities and T3 levels in commercial layer hens","volume":"2","author":"Melesse A.","year":"2011","journal-title":"International Journal of Livestock Production"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2011.10312"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nel116"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/86.8.1760"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2010-00921"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00484\u2010020\u201001887\u2010z"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1996.tb01830.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(98)75789-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(88)79834-3"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00102-0"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.5.1106"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01963.x"},{"key":"e_1_2_9_37_1","first-page":"90","article-title":"Estimating egg production declines at high environmental temperatures and humidities","author":"Zulovich J. 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