{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:44:36Z","timestamp":1762177476727,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T00:00:00Z","timestamp":1639008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The oocyte maturation process requires a high supply of energy, which generates reactive oxygen species (ROS), adversely affecting oocyte and embryo development. Balancing ROS by antioxidant supplementation is essential for maintaining oocyte maturation and embryonic quality in vitro. This study aimed to evaluate the impact of four antioxidants: \u03b2\u2010mercaptoethanol (\u03b2\u2010ME), chlorogenic acid (CGA), curcumin and sericin, when applied individually or in combinations, during oocyte maturation on development of porcine oocytes. Cumulus\u2013oocyte complexes were collected, cultured in maturation medium supplemented with antioxidants for 44\u00a0hr and subsequently subjected to in vitro fertilization (IVF) and culture for 7\u00a0days. Combining all four (\u03b2\u2010ME\u00a0+\u00a0CGA\u00a0+\u00a0curcumin\u00a0+\u00a0sericin) or three (\u03b2\u2010ME\u00a0+\u00a0CGA\u00a0+\u00a0curcumin) antioxidants increased blastocyst formation rates. However, sericin supplementation alone, or in combination with \u03b2\u2010ME or CGA, failed to improve blastocyst formation rates. The total cell numbers of blastocysts from the group supplemented with three antioxidants (\u03b2\u2010ME\u00a0+\u00a0CGA\u00a0+\u00a0curcumin) were significantly higher than those from the other groups, except for the curcumin\u2010supplement group. There were no differences in the maturation rates and proportions of oocytes with fragmented DNA between the antioxidant\u2010supplemented and the non\u2010supplemented control groups. In conclusion, supplementation with three antioxidants (\u03b2\u2010ME\u00a0+\u00a0CGA\u00a0+\u00a0curcumin) during the maturation culture enhanced blastocyst formation and improved blastocyst quality.<\/jats:p>","DOI":"10.1111\/rda.14063","type":"journal-article","created":{"date-parts":[[2021,12,4]],"date-time":"2021-12-04T14:30:27Z","timestamp":1638628227000},"page":"314-320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effects of individual or in\u2010combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5614-5570","authenticated-orcid":false,"given":"Chommanart","family":"Thongkittidilok","sequence":"first","affiliation":[{"name":"Faculty of Bioscience and Bioindustry Tokushima University Tokushima Japan"},{"name":"Bio\u2010Innovation 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