{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:35:23Z","timestamp":1785436523918,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T00:00:00Z","timestamp":1642377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["CVZ\u2010PPM 00201\u201017"],"award-info":[{"award-number":["CVZ\u2010PPM 00201\u201017"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9\u2010day (G\u20109<jats:sub>SOV<\/jats:sub>, <jats:italic>n<\/jats:italic>\u00a0=\u00a021) or 12\u2010day (G\u201012<jats:sub>SOV<\/jats:sub>, <jats:italic>n<\/jats:italic>\u00a0=\u00a021) progesterone (P<jats:sub>4<\/jats:sub>)\u2010based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle\u2010stimulating hormone (133\u00a0mg of pFSH given over 3\u00a0days). Non\u2010surgical embryo recovery (NSER) was performed 6\u20137\u00a0days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (<jats:italic>p\u00a0&lt;<\/jats:italic>\u00a0.05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G\u20109<jats:sub>SOV<\/jats:sub> and G\u201012<jats:sub>SOV<\/jats:sub> respectively. NSER was successfully completed in 86.2% of ewes that had CL (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The mean number of CL per ewe\/successfully flushed donor ewe was greater (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in G\u201012<jats:sub>SOV<\/jats:sub> (12.3\u00a0\u00b1\u00a01.7\/12.1\u00a0\u00b1\u00a01.9) than in G\u20109<jats:sub>SOV<\/jats:sub> (7.9\u00a0\u00b1\u00a01.4\/8.2\u00a0\u00b1\u00a01.6). Mean numbers of retrieved blastocysts and viable embryos were greater (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) in G\u201012<jats:sub>SOV<\/jats:sub> (5.8\u00a0\u00b1\u00a01.9 and 3.7\u00a0\u00b1\u00a01.7) than G\u20109<jats:sub>SOV<\/jats:sub> (3.5\u00a0\u00b1\u00a01.1 and 0.8\u00a0\u00b1\u00a00.3 respectively). The total follicle count (all follicles \u22652\u00a0mm in diameter) at the sixth pFSH dose (at P<jats:sub>4<\/jats:sub>\u2010device removal) was positively correlated (<jats:italic>p\u00a0&lt;<\/jats:italic>\u00a0.05) with the number of CL (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.95) and viable embryos (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.91) in G\u201012<jats:sub>SOV<\/jats:sub>. The ewes with \u226510 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G\u201012<jats:sub>SOV<\/jats:sub> group had better superovulatory responses compared with G\u20109<jats:sub>SOV<\/jats:sub> group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P<jats:sub>4<\/jats:sub> for 12\u00a0days; and (c) animals with \u226510 ovulations are main contributors to viable embryo production in Morada Nova ewes.<\/jats:p>","DOI":"10.1111\/rda.14077","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T14:55:24Z","timestamp":1640876124000},"page":"393-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non\u2010surgical uterine flushing"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7730-290X","authenticated-orcid":false,"given":"Maria Emilia Franco","family":"Oliveira","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista  Jaboticabal Brazil"},{"name":"Embrapa Caprinos e Ovinos  Sobral Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Matos","family":"Arrais","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o e Avalia\u00e7\u00e3o Animal Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Roberto Bourg","family":"de Mello","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o e Avalia\u00e7\u00e3o Animal Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Brun","family":"Vergani","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista  Jaboticabal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3242-2294","authenticated-orcid":false,"given":"Lucas Machado","family":"Figueira","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria Universidade Federal Fluminense  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Novita","family":"Esteves","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste  S\u00e3o Carlos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f4nica Schinaider do Amaral","family":"Pereira","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste  S\u00e3o Carlos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3354-1474","authenticated-orcid":false,"given":"Alexandre Rossetto","family":"Garcia","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste  S\u00e3o Carlos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-9137","authenticated-orcid":false,"given":"Pawel Mieczyslaw","family":"Bartlewski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Ontario Veterinary College University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8028-3984","authenticated-orcid":false,"given":"Jeferson Ferreira","family":"da Fonseca","sequence":"additional","affiliation":[{"name":"Embrapa Caprinos e Ovinos  Sobral Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.06.020"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2020.0125"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2007.11.003"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.024"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.07.002"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678\u20104162\u20108428"},{"key":"e_1_2_10_8_1","unstructured":"Brebion P. &Cogni\u00e9 Y.(1989).Increased superovulation in the Ewe following 14 days of GnRH agonist pre\u2010treatment. 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