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In addition, RBP4 contributes to testicular homeostasis, including maintenance of spermatogenesis and synthesis of androgens that mediate their physiological functions through the androgen receptor. RBP4 in Sertoli cells regulates testosterone and dihydrotestosterone synthesis and secretion, although the mechanisms have yet to be revealed. In this study, we examined the expression and function of RBP4 in Sertoli cells isolated from Bactrian camels. qRT\u2010PCR analysis of various Bactrian camel tissues revealed high expression of RBP4 in the testis and epididymis. To examine RBP4 function, Sertoli cells isolated from testes were transfected with an RBP4 overexpression plasmid or RBP4\u2010targeting siRNA. RBP4 overexpression resulted in significant inhibition of transcription and translation of the steroidogenic enzymes 3\u03b2HSD and SRD5A1 concomitant with a significant decrease in androgen receptor expression and dihydrotestosterone secretion. Conversely, RBP4 knockdown significantly increased the expression of 3\u03b2HSD, SRD5A1 and androgen receptor and enhanced the secretion of dihydrotestosterone and testosterone. These data reveal a novel role for RBP4 in regulating steroid synthesis in Sertoli cells from Bactrian camels.<\/jats:p>","DOI":"10.1111\/rda.14081","type":"journal-article","created":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T09:08:30Z","timestamp":1641892110000},"page":"429-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["RBP4 regulates androgen receptor expression and steroid synthesis in Sertoli cells from Bactrian camels"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7964-9309","authenticated-orcid":false,"given":"Qi","family":"Wang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Gansu Agriculture University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yina","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingxu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-8727","authenticated-orcid":false,"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University  Lanzhou China"},{"name":"College of Life Science and Technology Gansu Agriculture University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.10.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0816"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu3040385"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod21.4.891"},{"issue":"1","key":"e_1_2_9_6_1","first-page":"103","article-title":"In vitro effects of genistein and daidzein on the activity of adrenocortical steroidogenic enzymes in mature female pigs","volume":"64","author":"Kaminska B.","year":"2013","journal-title":"Journal of Physiology and Pharmacology"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod32.1.173"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.022061"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240173"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0303\u20107207(00)00271\u20109"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.6.1445"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.09.010"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010121\u20101\u2010432"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0739\u20107240(97)00060\u2010x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2011.05.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011\u2010014\u20100205\u20103"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/112.10.1881"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod49.5.972"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.085811"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000145008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20102952(95)02093\u20104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010010\u20100268\u2010z"},{"issue":"4","key":"e_1_2_9_24_1","first-page":"180","article-title":"Histological & ultrastructural studies on the effect of Vitamin Amin A depletion & subsequent repletion with Vitamin Amin A on germ cells & Sertoli cells in rat testis","volume":"21","author":"Unni E.","year":"1983","journal-title":"Indian Journal of Experimental Biology"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod43.3.363"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601447"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005\u20100953"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13607"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.42.6.753"},{"key":"e_1_2_9_30_1","unstructured":"Zeng\u2010Kui L. 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