{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:23:31Z","timestamp":1787509411259,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:00:00Z","timestamp":1644278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004481","name":"University of Tehran","doi-asserted-by":"publisher","award":["7108017\/6\/39"],"award-info":[{"award-number":["7108017\/6\/39"]}],"id":[{"id":"10.13039\/501100004481","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The objective was to compare effects of encapsulated or free glutathione (GSH) on the quality of frozen\u2010thawed bull sperm. Ejaculates were collected via artificial vagina from six mature Holstein bulls once weekly for 6\u00a0weeks. All ejaculates had motility \u226570%, sperm concentration \u22651.0\u00a0\u00d7\u00a010\n                    <jats:sup>9<\/jats:sup>\n                    \/ml and \u226415% morphologically abnormal sperm. Each week, semen was pooled and diluted with lecithin\u2010based extenders containing various concentrations of encapsulated (E0, E1, E2.5 and E5\u00a0mM) or free (F0, F1, F2.5 and F5\u00a0mM) GSH, with total glutathione content determined before and after cryopreservation. Total GSH in fresh semen was (mean+\n                    <jats:italic>SEM<\/jats:italic>\n                    ) 4.8\u00a0\u00b1\u00a00.2\u00a0nmol\/10\n                    <jats:sup>8<\/jats:sup>\n                    sperm, whereas in frozen\u2010thawed semen of group F0 (control), it decreased to 1.4\u00a0\u00b1\u00a00.2\u00a0nmol\/10\n                    <jats:sup>8<\/jats:sup>\n                    sperm, a 70.8% reduction (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). In addition, total GSH in frozen\u2010thawed semen from groups E2.5, E5 and F5 were 2.4\u00a0\u00b1\u00a00.2, 2.8\u00a0\u00b1\u00a00.2 and 1.8\u00a0\u00b1\u00a00.2\u00a0nmol\/10\n                    <jats:sup>8<\/jats:sup>\n                    sperm, respectively (E5 versus. F0,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). Compared to group F0, frozen\u2010thawed sperm from group E2.5 had greater (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) percentages of sperm that were viable (Annexin\u2010V) (61.1\u00a0\u00b1\u00a01.8 versus. 71.1\u00a0\u00b1\u00a01.8) and that had cell membrane integrity (eosin\u2010nigrosin) (64.5\u00a0\u00b1\u00a03.1 versus. 80.0\u00a0\u00b1\u00a03.1). Furthermore, frozen\u2010thawed sperm from group E2.5 had the numerically highest total and progressive motility (CASA) and cell membrane functionality (HOS) and the lowest percentage of early apoptotic sperm (Annexin\u2010V). However, acrosome membrane integrity (PSA) of E5 had the lowest mean (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05), whereas E2.5 caused a small nonsignificant decrease (69.1\u00a0\u00b1\u00a01.4%) compared to E0 and F0. In conclusion, 2.5\u00a0mM encapsulated GSH in semen extender significantly improved the quality of frozen\u2010thawed bull sperm.\n                  <\/jats:p>","DOI":"10.1111\/rda.14088","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T20:43:32Z","timestamp":1643316212000},"page":"515-523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Supplementation of freezing medium with encapsulated or free glutathione during cryopreservation of bull sperm"],"prefix":"10.1111","volume":"57","author":[{"given":"Touba","family":"Nadri","sequence":"first","affiliation":[{"name":"Department of Animal Science College of Agriculture and Natural Resources University of Tehran  Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3288-1709","authenticated-orcid":false,"given":"Armin","family":"Towhidi","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture and Natural Resources University of Tehran  Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Zeinoaldini","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture and Natural Resources University of Tehran  Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamhossein","family":"Riazi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Biochemistry and biophysics (IBB) University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Sharafi","sequence":"additional","affiliation":[{"name":"Department of Embryology Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine ACECR  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2886-9747","authenticated-orcid":false,"given":"Mahdi","family":"Zhandi","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture and Natural Resources University of Tehran  Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4607-3355","authenticated-orcid":false,"given":"John","family":"Kastelic","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine University of Calgary  Calgary Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dariush","family":"Gholami","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology Amol University of Special Modern Technologies  Amol Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0860185"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(02)01369\u20109"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01973.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2017.002"},{"key":"e_1_2_9_6_1","first-page":"235","article-title":"Effect of exogenous glutathione in extender on the freezability of Nili\u2010Ravi buffalo (Bubalus bubalis) bull spermatozoa","volume":"28","author":"Ansari M. 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