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However, the role of folate receptors during pre\u2010implantation stages of embryos is not well known. Thus, the present study aimed to investigate the expression of folate transport genes and proteins in mature oocytes and pre\u2010implantation embryos and the effect of FOLR1 knockdown in zygotes on blastocyst outcome. For this, immature goat oocytes were matured in maturation medium followed by in vitro fertilization and culture at standard conditions. A group of zygotes was transfected with esiRNA against <jats:italic>FOLR1<\/jats:italic> and in vitro cultured for blastocyst outcome assessment. The transcripts and proteins for <jats:italic>FOLR1<\/jats:italic>, <jats:italic>FOLR2<\/jats:italic> and <jats:italic>RFC1<\/jats:italic> were present in oocytes as well as all the stages of pre\u2010implantation embryos. Immunofluorescence revealed the presence of FOLR1 in the nuclei of embryos but not in the metaphase (matured) oocytes. The knockdown of <jats:italic>FOLR1<\/jats:italic> in embryos was effective and significantly reduced the blastocyst production rate. The present study demonstrates the existence of active folate transport in oocytes and pre\u2010implantation goat embryos. FOLR1 is vital for pre\u2010implantation embryo development and may aid in the progression by functioning as a transcription factor.<\/jats:p>","DOI":"10.1111\/rda.14092","type":"journal-article","created":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T17:43:52Z","timestamp":1644083032000},"page":"541-549","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Folate receptor\u20101 is vital for developmental competence of goat embryos"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8112-0738","authenticated-orcid":false,"given":"Sikander","family":"Saini","sequence":"first","affiliation":[{"name":"Animal Biotechnology Centre National Dairy Research Institute  Karnal 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