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Forty\u2010four multiparous mix\u2010breed Murrah buffaloes were selected for the study. The cows were subjected to hormonal oestrous synchronization and separated into 4 groups, P12 (pregnant, <jats:italic>n<\/jats:italic>\u00a0=\u00a08) and P18 (<jats:italic>n<\/jats:italic>\u00a0=\u00a08) at 12 and 18\u00a0days post\u2010insemination; NP12 (non\u2010pregnant, <jats:italic>n<\/jats:italic>\u00a0=\u00a07) and NP18 (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) at 23 and 29\u00a0days after the onset of synchronization, respectively. The embryos and blood were collected, and the plasma was separated for centrifugation and used to determine progesterone concentration. Progesterone concentration was higher in P18 than P12 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.02) and NP18 groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001). The steroid was also increased in the P12 group compared with NP12 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.031). There was no difference between NP12 and NP18 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.906). We conclude that the plasma progesterone concentration can be an alternative to identify earlier non\u2010pregnant buffaloes, advancing the oestrous resynchronization or natural service to improve productivity.<\/jats:p>","DOI":"10.1111\/rda.14108","type":"journal-article","created":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:54:42Z","timestamp":1646484882000},"page":"673-677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Can blood progesterone concentration identify non\u2010pregnant buffaloes to support oestrous resynchronization?"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0528-5124","authenticated-orcid":false,"given":"Viviane M.","family":"Codognoto","sequence":"first","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4721-1801","authenticated-orcid":false,"given":"Fabiana F.","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4420-469X","authenticated-orcid":false,"given":"Let\u00edcia C.","family":"Salgado","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9423-9658","authenticated-orcid":false,"given":"Guilherme","family":"Rizzoto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6672-5547","authenticated-orcid":false,"given":"Paulo H.","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nayara F. S.","family":"Marques","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2867-3648","authenticated-orcid":false,"given":"N\u00e9lcio A. T.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Unidade de Pesquisa e Desenvolvimento de Registro P\u00f3lo Regional do Vale do Ribeira (APTA)  Registro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariane","family":"Dantas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Vict\u00f3ria Pereira","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7742-8254","authenticated-orcid":false,"given":"Jo\u00e3o C. 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