{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:32Z","timestamp":1787015972842,"version":"build-2736575974"},"reference-count":9,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Contents<\/jats:title>\n                  <jats:p>\n                    Present study aimed to evaluate field fertility rate and calf sex ratio of Nili Ravi buffalo semen sexed through modified swim\u2010up method (Animal Reproduction Science, 182, 2017, 69). For this purpose, five mature Nili\u2010Ravi buffalo bulls kept at semen production unit, Qadirabad, Pakistan, were selected. Two consecutive ejaculates per week were collected with artificial vagin\u0105 for 3\u00a0weeks. Qualified semen ejaculates were pooled and divided into two aliquots. The first aliquot was processed by routine procedure (control), whereas the second was processed by modified swim\u2010up technique. After separation, semen was diluted in\n                    <jats:italic>tris<\/jats:italic>\n                    \u2010citric acid extender and cryopreserved using standard techniques. Sexed semen was evaluated for fertility trials during peak breeding season. Artificially inseminated animals were examined for pregnancy rate through rectal palpation at least 3\u00a0months after insemination under field conditions. Calving ratio of female and male calves were recoded after Parturition. The fertility rate was higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) in X\u2010sorted sperm (70%) as compared with control (47%). The female calf ratio was higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) in X\u2010sorted sperm (78.58%) compared with control (53.3%). In Conclusion, conception rate and production of female calf were significantly higher with sexed semen separated through modified swim\u2010up method compared with unsexed control.\n                  <\/jats:p>","DOI":"10.1111\/rda.14112","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T13:15:20Z","timestamp":1647522920000},"page":"798-801","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pregnancy and calving rates improved using modified swim\u2010up method for buffalo semen sexing"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4136-1764","authenticated-orcid":false,"given":"Asma Ul","family":"Husna","sequence":"first","affiliation":[{"name":"Department of Biology (Zoology) University of Haripur  Haripur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4446-1875","authenticated-orcid":false,"given":"Asima","family":"Azam","sequence":"additional","affiliation":[{"name":"Department of Zoology Shaheed Benazir Bhutto Women University Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Qadeer","sequence":"additional","affiliation":[{"name":"Department of Zoology Division of Science and Technology University of Education  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7313-526X","authenticated-orcid":false,"given":"Rabea","family":"Ejaz","sequence":"additional","affiliation":[{"name":"Department of Zoology Shaheed Benazir Bhutto Women University Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamim","family":"Akhter","sequence":"additional","affiliation":[{"name":"Department of Zoology Pir Mehr Ali Shah Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12252"},{"key":"e_1_2_10_3_1","first-page":"259","article-title":"Sperm sexing and its application in livestock sector","volume":"7","author":"Bhalakiya N.","year":"2018","journal-title":"International Journal of Current Microbiology and Applied Sciences"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.04.011"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.04.011"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2004.04.013"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.09.027"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(97)00368\u20103"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(04)70067\u20103"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.09.012"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14112","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.14112","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T22:29:02Z","timestamp":1692829742000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,25]]},"references-count":9,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/rda.14112"],"URL":"https:\/\/doi.org\/10.1111\/rda.14112","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,25]]},"assertion":[{"value":"2022-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}