{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T22:15:38Z","timestamp":1768083338483,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Overproduction of reactive oxygen species (ROS) during sperm cryopreservation has a detrimental effect on sperm parameters. Therefore, the use of antioxidants in the sperm freezing extender can reduce ROS destructive effects. In this study, we investigated whether co\u2010supplementation of melatonin and myo\u2010inositol into the semen extender can improve the post\u2010cryopreservation quality of goat spermatozoa. After the freeze\u2013thawing process, sperm motility, viability, plasma membrane and acrosome intact morphology were improved in the combined myo\u2010inositol and melatonin group compared to both individual and the control groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In addition, the mean of sperm ROS, DNA damage and lipid peroxidation were reduced in co\u2010supplementation of myo\u2010inositol and melatonin compared to their individual counterparts (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Therefore, the synergistic effects of myo\u2010inositol and melatonin on the cryopreserved spermatozoa are highly likely mediated through the reduction in important factors involved in the sperm lipid peroxidation. Finally, we used the cryopreserved spermatozoa for in vitro production of embryos. Results showed that combined group of myo\u2010inositol and melatonin improved the cleavage rate compared to both individual and control groups, although blastocyst rate was improved using both individual and combined groups. In conclusion, co\u2010supplementation of melatonin and myo\u2010inositol is a promising approach for the improvement of goat sperm cryopreservation.<\/jats:p>","DOI":"10.1111\/rda.14131","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T09:35:37Z","timestamp":1650879337000},"page":"876-885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Synergistic effects of myo\u2010inositol and melatonin on cryopreservation of goat spermatozoa"],"prefix":"10.1111","volume":"57","author":[{"given":"Nima","family":"Tanhaei Vash","sequence":"first","affiliation":[{"name":"Department of Biology, Cellular and Developmental Biology Payame Noor University  Isfahan Iran"},{"name":"Department of Animal Biotechnology Reproductive Biomedicine Research Center Royan Institute for Biotechnology ACECR  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parisa","family":"Nadri","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture Isfahan University of Technology  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3033-981X","authenticated-orcid":false,"given":"Akbar","family":"Karimi","sequence":"additional","affiliation":[{"name":"Department of Biology, Cellular and Developmental Biology Payame Noor University  Isfahan Iran"},{"name":"Department of Biology Payame Noor University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/smj.2017.40894"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2017.09.006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2019.05.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.03.016"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB11.1020"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010079X.2011.00873.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00014.2009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2012.05.011"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u20107\u2010125"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12259"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2010.09.001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.224"},{"key":"e_1_2_10_14_1","first-page":"617","article-title":"Supplementation of a commercial extender (BTS) with melatonin to improve quality of boar semen in cooling storage at 15o C","volume":"42","author":"Chankitisakul V.","year":"2014","journal-title":"Khon Kaen Agriculture Journal"},{"key":"e_1_2_10_15_1","first-page":"891","article-title":"Inositol activity in oligoasthenoteratospermia\u2013An in vitro study","volume":"14","author":"Colone M.","year":"2010","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_10_16_1","first-page":"129","article-title":"Effects of myoinositol on sperm mitochondrial function in\u2010vitro","volume":"15","author":"Condorelli R.","year":"2011","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_10_17_1","first-page":"139","article-title":"The hypoosmotic swelling test in fresh goat spermatozoa. 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