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For this purpose, epididymal sperm from six sexually mature male agoutis were collected via flotation. Then, analyses of sperm parameters and hypoosmotic swelling test using different hypoosmotic solutions (0, 50 and 200\u00a0mOsm\/L) in different regions of the epididymis (caput, corpus and cauda) were performed. There was an increase (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in the values for sperm concentration, the total number of sperm recovered, total and progressive motility, average path velocity, straight\u2010line velocity, curvilinear velocity, and rapid and medium subpopulations following the caput\u2010corpus\u2010cauda direction. Regardless of the hypoosmotic solution, the agouti sperm membrane presented similar functional integrity in all the epididymal regions. Moreover, the highest (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) osmotic responses were reached with the use of 50\u00a0mOsm\/L solution in comparison to 0 and 200\u00a0mOsm\/L for all the regions. Significant correlations among osmotic response and some sperm kinetic parameters were observed, especially in epididymal caput, while no correlations were found in the region of the cauda. In summary, red\u2010rumped agouti sperm present similar membrane functionality during epididymal transit, but there are evident correlations among such functionality and sperm kinetic parameters, especially in the caput region. Moreover, we indicate the use of a 50\u00a0mOsm\/L hypoosmotic solution for the analysis of this parameter through the hypoosmotic swelling test.<\/jats:p>","DOI":"10.1111\/rda.14140","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T19:28:43Z","timestamp":1651519723000},"page":"912-918","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of sperm membrane functionality during epididymal transit in red\u2010rumped agouti (<i>Dasyprocta leporina<\/i>)"],"prefix":"10.1111","volume":"57","author":[{"given":"Maiko Roberto Tavares","family":"Dantas","sequence":"first","affiliation":[{"name":"Laboratory on Animal Germplasm Conservation Universidade Federal Rural do Semi\u2010\u00c1rido \u2013 UFERSA  Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayra Rachel Nascimento","family":"Luz","sequence":"additional","affiliation":[{"name":"Laboratory on Animal Germplasm Conservation Universidade Federal Rural do Semi\u2010\u00c1rido \u2013 UFERSA  Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Grasiele Pereira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory on Animal Germplasm Conservation Universidade Federal Rural do Semi\u2010\u00c1rido \u2013 UFERSA  Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samara Sandy Jer\u00f4nimo","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laboratory on Animal Germplasm Conservation Universidade Federal Rural do Semi\u2010\u00c1rido \u2013 UFERSA  Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Franco","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences Universidade Federal Rural do Semi\u2010\u00c1rido \u2013 UFERSA  Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory on Animal Germplasm Conservation Universidade Federal Rural do Semi\u2010\u00c1rido \u2013 UFERSA  Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.05.006"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678\u20104162\u201010452"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.09.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12517"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2006.00810.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12007"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.29327\/multiscience.2022014"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201008\u20100225"},{"key":"e_1_2_9_10_1","unstructured":"Emmons L. &Reid F.(2016).Dasyprocta leporina. 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