{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T18:02:24Z","timestamp":1772733744387,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011093","name":"University of Phayao","doi-asserted-by":"publisher","award":["FF64\u2010UoE010"],"award-info":[{"award-number":["FF64\u2010UoE010"]}],"id":[{"id":"10.13039\/501100011093","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011093","name":"University of Phayao","doi-asserted-by":"publisher","award":["RD63100"],"award-info":[{"award-number":["RD63100"]}],"id":[{"id":"10.13039\/501100011093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Egg yolk is a common cryoprotectant that can be used as a semen extender to protect the spermatozoa from damage during cryopreservation. Therefore, the aim of this study was to investigate the efficacy of fresh and lyophilized egg yolk, as a Tris\u2010base extender, on the quality of cryopreserved goat semen. Semen from 10 rams of two different breeds (Boer and Saanen) was collected using an artificial vagina. Each ejaculate sample was divided into four equal aliquots, which contained 20% of the fresh egg yolk (a control group), and then 10%, 15%, and 20% of the lyophilized egg yolk as a Tris\u2010base extender. Sperm motility and kinetic parameters were determined using a computer\u2010assisted semen analyser. The results showed that the addition of 20% of the fresh egg yolk in Tris\u2010base extender exhibited significantly higher progressive motility, progressive fast motility, distance curve line, and beat\u2010cross frequency parameters in the post\u2010thaw Boer and Saanen goat sperm when compared with the addition of 10%, 15%, and 20% of the lyophilized egg yolk. The percentage of total motility and immotile parameters in the post\u2010thaw Boer and Saanen goat sperm were not significantly different between the control and 10%, 15% as well as 20% of the lyophilized egg yolk groups. Moreover, the percentage of viability parameter in the Boer and Saanen goat sperm was not significantly different between the control and 10% of the lyophilized egg yolk group but showed significant difference between the control group and 15% and 20% of the lyophilized egg yolk groups. Furthermore, the interaction between the two breeds was significantly different in terms of head activity and straightness parameter. In conclusion, the treatment with 20% of fresh egg yolk in Tris\u2010base extender is superior to the lyophilized egg yolk. However, an addition of 10% of the lyophilized egg yolk in Tris\u2010base extender presented the percentage of total motility and viability parameters showing no difference with 20% of fresh egg yolk. Therefore, 10% of the lyophilized egg yolk in Tris\u2010base extender provided detail of the lyophilized egg yolk protocol in cryopreserved goat semen as an example of an alternative extender to 20% of fresh egg yolk for situations where an animal's origin represents a microbiological risk.<\/jats:p>","DOI":"10.1111\/rda.14155","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T07:44:23Z","timestamp":1652427863000},"page":"928-935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The replacement of fresh egg yolk by lyophilized egg yolk in Tris\u2010base extender in cryopreserved Boer and Saanen semen"],"prefix":"10.1111","volume":"57","author":[{"given":"Payungsuk","family":"Intawicha","sequence":"first","affiliation":[{"name":"Division of Animal Science School of Agriculture and Natural Resources, University of Phayao  Phayao Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tossapol","family":"Moonmanee","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences Faculty of Agriculture Chiangmai University  Chiangmai Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sureeporn","family":"Saengwong","sequence":"additional","affiliation":[{"name":"Division of Animal Science School of Agriculture and Natural Resources, University of Phayao  Phayao Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chompunut","family":"Lumsangkul","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences Faculty of Agriculture Chiangmai University  Chiangmai Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Jing","family":"Liao","sequence":"additional","affiliation":[{"name":"Division of Physiology Livestock Research Institute, Council of Agriculture, Executive Yuan  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0176-8009","authenticated-orcid":false,"given":"Wilasinee","family":"Inyawilert","sequence":"additional","affiliation":[{"name":"Department of Agricultural Science Faculty of Agriculture Natural Resources and Environment, Naresuan University  Phitsanulok Thailand"},{"name":"The center for Agricultural Biotechnology Naresuan University  Phitsanulok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.03.007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.08.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2003.10.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2017.521\u2010525"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921\u20104488(01)00189\u20104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03268.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2006.00623.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20565"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(98)00036\u20103"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10080806"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.10.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5958\/0973\u20109718.2016.00005.2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(97)00136\u20102"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2009-2335"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13851"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4141\/a03\u2010024"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.05.010"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104320(00)00156\u20101"},{"issue":"4","key":"e_1_2_9_20_1","first-page":"186","article-title":"Correlation between hypo\u2010osmotic swelling test and various conventional semen EVALUATION parameters in fresh NILI\u2010RAVI buffalo and SAHIWAL cow bull semen","volume":"28","author":"Lodhi L.","year":"2008","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10061101"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2018.0113"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106315"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.08.016"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13063"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.06.018"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-072169323-1.50071-4"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2010.06.002"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2005.02.015"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.17582\/journal.vsrr\/2020\/6.1.46.57"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13092"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2019.12.004"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.06.016"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14155","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.14155","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14155","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T10:13:31Z","timestamp":1692785611000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,25]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1111\/rda.14155"],"URL":"https:\/\/doi.org\/10.1111\/rda.14155","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,25]]},"assertion":[{"value":"2022-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}