{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:31:29Z","timestamp":1788492689170,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"S5","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007480","name":"Universidad de Castilla-La Mancha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007480","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007480","name":"Universidad de Castilla-La Mancha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007480","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2017\u201089017\u2010R"],"award-info":[{"award-number":["AGL2017\u201089017\u2010R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Incubation with estrous sheep serum (ESS) is required to induce in vitro capacitation of spermatozoa during in vitro fertilization of small ruminants. However, the effect of adding different serum concentrations in the fertilization media on the quality of resulting blastocysts has not yet been studied. Here, 298 sheep oocytes were co\u2010incubated with capacitated spermatozoa with either 10% or 2% ESS. There were no differences between treatments in cleavage (10% ESS: 63.81\u2009\u00b1\u20095.87% and 2% ESS: 45.31\u2009\u00b1\u20095.87%) and blastocyst rates (10% ESS: 20.83\u2009\u00b1\u20092.12% and 2% ESS: 15.93\u2009\u00b1\u20092.12%). Nonetheless, in vitro\u2010produced blastocysts from the 10% ESS treatment showed a higher transcript abundance of mRNAs involved in apoptosis (<jats:italic>ITM2B<\/jats:italic> and <jats:italic>BCL2<\/jats:italic>), antioxidant defence (<jats:italic>GPX1<\/jats:italic>) and growth\u2010related imprinting (<jats:italic>IGF2R<\/jats:italic>). Our data suggest that ESS supplementation during in vitro fertilization can influence the quality of sheep embryos at later stages of development by increasing the transcription of developmentally important genes.<\/jats:p>","DOI":"10.1111\/rda.14161","type":"journal-article","created":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T14:09:27Z","timestamp":1652710167000},"page":"68-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serum supplementation during in vitro fertilization of sheep oocytes influences blastocyst quality through the differential abundance of <scp>mRNA<\/scp> transcripts"],"prefix":"10.1111","volume":"57","author":[{"given":"Irene","family":"S\u00e1nchez\u2010Ajofr\u00edn","sequence":"first","affiliation":[{"name":"Instituto Regional de Investigaci\u00f3n Cient\u00edfica Aplicada (IRICA) UCLM  Ciudad Real Spain"},{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6266-0635","authenticated-orcid":false,"given":"Patricia","family":"Peris\u2010Frau","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete Spain"},{"name":"Department Animal Reproduction (INIA\u2010CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Garc\u00eda\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Roc\u00edo","family":"Fern\u00e1ndez\u2010Santos","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidal","family":"Montoro","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3667-6518","authenticated-orcid":false,"given":"Jos\u00e9 Juli\u00e1n","family":"Garde","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-5157","authenticated-orcid":false,"given":"Ana Josefa","family":"Soler","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC\u2010UCLM\u2010JCCM) ETSIAM  Albacete 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in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.14161","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T01:32:46Z","timestamp":1692667966000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,31]]},"references-count":15,"journal-issue":{"issue":"S5","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/rda.14161"],"URL":"https:\/\/doi.org\/10.1111\/rda.14161","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,31]]},"assertion":[{"value":"2022-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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