{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:46:15Z","timestamp":1790664375365,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T00:00:00Z","timestamp":1655164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009587","name":"School of Veterinary Science, Shiraz University","doi-asserted-by":"publisher","award":["98GCB2M369962"],"award-info":[{"award-number":["98GCB2M369962"]}],"id":[{"id":"10.13039\/100009587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cauda epididymis holds a collectible source of fertile spermatozoa in cases of obstructive azoospermia, sudden death, and after elective or emergency castration. The current study was conducted to compare three different epidydimal sperm collection methods (Percutaneous epididymal sperm aspiration (PESA), microsurgical epididymal sperm aspiration (MESA) and retrograde epididymal wash (EW)) in the dog. Fifteen large\u2010breed adult dogs were applied for comparing the PESA (left testicles) with MESA (right testicles) techniques, while five dogs were used for evaluation of MESA (left testicles) versus EW (right testicles). The recovered sperm cells from MESA and EW were subjected to cryopreservation. Total sperm recovery, level of blood contamination and sperm quality markers (viability, morphology, plasma and acrosome membrane integrity, DNA fragmentation, and metabolic activity) were evaluated for fresh and frozen\u2013thawed spermatozoa. We showed that the collection of epididymal sperm cells through the PESA method resulted in lower total sperm recovery and significantly reduced fresh sperm kinematic and quality measures. While, both MESA and EW procedures resulted in a high number of intact epididymal spermatozoa with appropriate cryo\u2010tolerance potential. In conclusion, EW and MESA methods provide high\u2010quality epidydimal spermatozoa with high cryopreservation potential in domestic dogs.<\/jats:p>","DOI":"10.1111\/rda.14171","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T07:05:30Z","timestamp":1654585530000},"page":"1038-1045","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effects of epididymal sperm recovery methods on fresh and frozen\u2013thawed sperm characteristics in dogs"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3136-2288","authenticated-orcid":false,"given":"Asghar","family":"Mogheiseh","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, School of Veterinary Medicine Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-429X","authenticated-orcid":false,"given":"Mohammad Reza","family":"Divar","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, School of Veterinary Medicine Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser","family":"Vesal","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, School of Veterinary Medicine Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Mahdivand\u00a0Moradloo","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, School of Veterinary Medicine Shiraz University  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00278-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5455\/OVJ.2021.v11.i2.9"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03186-8"},{"key":"e_1_2_11_5_1","volume-title":"Small animal surgery textbook\u2010E\u2010book","author":"Fossum T. W.","year":"2013"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00090-2"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0421"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.66.37"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0241"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2021.07.003"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-52057-0"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-1591.78439"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-5200-2"},{"key":"e_1_2_11_14_1","unstructured":"Varesi S.(2012).Canine epididymal spermatozoa: Characteristics collection and cryopreservation. Philosophy of Doctor Thesis\u2010Veterinary Clinical Sciences\u2010Universit\u00e0 degli Studi di Milano."},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12020"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-55-17"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.22233\/9781905319541.8"},{"issue":"5","key":"e_1_2_11_18_1","first-page":"7475","article-title":"Repetitive percutaneous epididymal sperm aspirations (PESA's) resulted in asthenospermia and significant inflammation","volume":"21","author":"Zhang Y.","year":"2014","journal-title":"The Canadian Journal of Urology"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14171","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.14171","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T12:00:44Z","timestamp":1692792044000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,14]]},"references-count":17,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1111\/rda.14171"],"URL":"https:\/\/doi.org\/10.1111\/rda.14171","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,14]]},"assertion":[{"value":"2021-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}