{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:51:30Z","timestamp":1791571890817,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["436382\/2018\u20100"],"award-info":[{"award-number":["436382\/2018\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["405312\/2021\u20100"],"award-info":[{"award-number":["405312\/2021\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pyometra is one of the most common diseases in adult female dogs, characterized by a suppurative bacterial infection of the uterus with accumulation of inflammatory exudate and a variety of local and systemic clinical manifestations. This study aimed to identify the bacteria within the uterine content and vaginal canal of bitches with pyometra and evaluate their antimicrobial susceptibility and production of virulence factors. Uterine and vaginal content were collected with sterile swabs from 30 bitches diagnosed with pyometra. Bacteria were identified and assessed for their antimicrobial susceptibility and production of virulence factors, including biofilms, siderophores, proteases and hemolysins, both in planktonic and biofilm forms. A total of 82 bacterial isolates (35 uterus, 47 vagina), belonging to 21 species, were identified, with\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    as the most prevalent species (32\/82, 39%). As for susceptibility, 39\/79 (49.4%) isolates were resistant to one or more drugs, with resistance proportion among Gram\u2010positive bacteria (87.5%) higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05) than that observed for Gram\u2010negative bacteria (32.7%). Four coagulase\u2010negative\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    species were resistant to methicillin. Regarding virulence, the isolates had low production of biofilms, siderophores, proteases and hemolysins, suggesting that the occurrence of pyometra might be more associated with host\u2010related factors than bacterial virulence.\n                  <\/jats:p>","DOI":"10.1111\/rda.14181","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T11:54:58Z","timestamp":1655121298000},"page":"1063-1073","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Antimicrobial susceptibility and production of virulence factors by bacteria recovered from bitches with pyometra"],"prefix":"10.1111","volume":"57","author":[{"given":"Marcos F. G.","family":"Rocha","sequence":"first","affiliation":[{"name":"Specialized Medical Mycology Center, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"},{"name":"Post\u2010Graduate Program in Veterinary Sciences, School of Veterinary State University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9bora D. Q.","family":"Paiva","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno R.","family":"Amando","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carliane M. A.","family":"Melgarejo","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyne S.","family":"Freitas","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco I. F.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crister J.","family":"Ocadaque","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia L.","family":"Costa","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glaucia M. M.","family":"Guedes","sequence":"additional","affiliation":[{"name":"Group of Applied Medical Microbiology, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reginaldo G.","family":"Lima\u2010Neto","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Health Sciences Center Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana A.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, Post\u2010Graduate Program in Medical, Department of Pathology and Legal Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 J. 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