{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T06:05:27Z","timestamp":1769321127187,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T00:00:00Z","timestamp":1658793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001655","name":"Deutscher Akademischer Austauschdienst","doi-asserted-by":"publisher","award":["57552395"],"award-info":[{"award-number":["57552395"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study evaluated the effect of different proportions of dead spermatozoa on the quality of liquid boar semen during a thermo\u2010resistance test (TRT). After 3\u2009days of storage (17\u00b0C), 54 conventional artificial insemination semen doses (~23\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> sperm\/ml in ~88\u2009ml of BTS) were split into three 15\u2009ml\u2010treatments (25%, 50%, and 75% dead sperm cells) by mixing two subsamples containing 75% (I) and 0% (II) of live cells. Spermatozoa were evaluated after TRT at 30 (on\u2010test) and 300\u2009min (off\u2010test) incubation at 38\u00b0C. At the on\u2010test, treatments of 25%, 50%, and 75% dead sperm cells showed medians for total sperm motility of 77.6%, 50.2%, and 25.6%, respectively. Considering the absolute variation of sperm motility during TRT, doses with 25% dead sperm lost more percentage points (pp) (\u22129.4\u00a0pp) compared to doses containing 50% (\u22128.2\u00a0pp) and 75% dead sperm (\u22124.5\u00a0pp). The lowest loss was observed for doses with 75% dead sperm (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). However, data showed that treatments lost similar proportion of motile cells over the TRT: 25% dead sperm\u00a0=\u00a0\u221211.9%, 50% dead sperm\u00a0=\u00a0\u221216.0%, and 75% dead sperm\u00a0=\u00a0\u221217.5% (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.31). Regarding the flow cytometry parameters (plasma and acrosomal membrane integrity, mitochondrial activity of cells with intact plasma membrane, high degree of lipid disorder, and apoptotic cells), the absolute variations did not surpass values of \u22121.8, 3.4, \u22125.4, and 4.7\u00a0pp, respectively. Furthermore, the relative variation suggested that dead sperm did not substantially change their values over the TRT. In conclusion, dead sperm cells did not influence the quality of contemporary live cells during the period and in conditions of a TRT.<\/jats:p>","DOI":"10.1111\/rda.14208","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T13:38:44Z","timestamp":1658151524000},"page":"1327-1335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Live cells are not affected by dead sperm in liquid boar semen: New insights based on a thermo\u2010resistance test"],"prefix":"10.1111","volume":"57","author":[{"given":"Monike","family":"Quirino","sequence":"first","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"},{"name":"Setor de Su\u00ednos, Faculdade de Veterin\u00e1ria Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Jakop","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula Gon\u00e7alves","family":"Mellagi","sequence":"additional","affiliation":[{"name":"Setor de Su\u00ednos, Faculdade de Veterin\u00e1ria Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-328X","authenticated-orcid":false,"given":"Fernando\u00a0Pandolfo","family":"Bortolozzo","sequence":"additional","affiliation":[{"name":"Setor de Su\u00ednos, Faculdade de Veterin\u00e1ria Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Jung","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7932-9412","authenticated-orcid":false,"given":"Martin","family":"Schulze","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.12.2797"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.5.1531"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(02)00941\u2010X"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.11.016"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.02.035"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0264675"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019069"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1642\u2010431X(12)60062\u2010X"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2527\/2003.81112818x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.12.029Get"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036550"},{"key":"e_1_2_10_13_1","volume-title":"Assessment of sperm and oxidative stress parameters upon homogenization of liquid\u2010stored boar semen in different extenders","author":"Menegat M. 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