{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:52:38Z","timestamp":1787161958844,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T00:00:00Z","timestamp":1661644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The current study examines the protective effects of different melatonin concentrations on fresh ram epididymis spermatozoa after post\u2010mortem recovery under normal and oxidative stress conditions and during liquid preservation (4\u00b0C) at different times (24, 48 and 72\u2009h). The testes were obtained from a local slaughterhouse during the breeding season. Spermatozoa were isolated from cauda epididymides. In experiment 1, the effects of adding different concentrations of melatonin (0, 15, 60 and 240\u2009\u03bcg\/ml) under normal and oxidative stress conditions were evaluated. Fifty \u03bcM of hydrogen peroxide was used to induce oxidative stress. Also, in experiment 2, the spermatozoa samples were chilled to 4\u00b0C and stored for 72\u2009h. Sperm total motility, viability, membrane, DNA integrity and morphological abnormality were evaluated at 0, 24, 48 and 72\u2009h after cooling storage. In experiment 1, melatonin treatment preserved viability increased TAC and SOD activities, and reduced MDA levels compared with control. Also, melatonin reduced the harmful effects of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    under induced oxidative stress. In experiment 2, melatonin at concentrations of 15 and 60\u2009g\/ml greatly increased sperm viability after 3\u2009days of cold storage. Furthermore, it could have a significant protective effect on the motility of cooled sperm. Melatonin supplementation preserved higher sperm membrane integrity at concentrations of 15 and 60\u2009\u03bcg\/ml, DNA integrity at a concentration of 15\u2009\u03bcg\/ml and abnormality at a concentration of 60\u2009\u03bcg\/ml after 3\u2009days of storage. The results suggest that melatonin can be used to reduce the adverse effects of induced oxidative stress in spermatozoa. Furthermore, ram epididymal spermatozoa could be stored at 4\u00b0C for 72\u2009h when treated with melatonin.\n                  <\/jats:p>","DOI":"10.1111\/rda.14228","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T11:19:56Z","timestamp":1660821596000},"page":"1520-1528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of different concentrations of melatonin on ram epididymal spermatozoa recovered post\u2010mortem under oxidative stress conditions and storage at 4\u00b0C"],"prefix":"10.1111","volume":"57","author":[{"given":"Mohsen","family":"Shayestehyekta","sequence":"first","affiliation":[{"name":"Basic Sciences and Pathobiology Department, Faculty of Veterinary Medicine Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayebeh","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Basic Sciences and Pathobiology Department, Faculty of Veterinary Medicine Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5007-5110","authenticated-orcid":false,"given":"Leila","family":"Soltani","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, College of Agriculture and Natural Resources Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrdad","family":"PooyanMehr","sequence":"additional","affiliation":[{"name":"Basic Sciences and Pathobiology Department, Faculty of Veterinary Medicine Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-017-0884-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u201012\u2010112"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2017.35.2.77"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1002\/j.1939-4640.1992.tb00306.x","article-title":"Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a model of sublethal cryo damage to human sperm during cryopreservation","volume":"13","author":"Alvarez J. 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