{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:24:56Z","timestamp":1777519496554,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T00:00:00Z","timestamp":1662336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study aimed to validate the double versus single freezing protocol for Beetal buck (<jats:italic>Capra hircus<\/jats:italic>) spermatozoa in tris\u2010citric acid (TCA) based extender both in terms of quality and fertilization potential. Computer\u2010assisted sperm motion and kinematic (CASA) variables, i.e. total (%), and progressive motilities (TM and PM, %) and rapid velocity (RV, %), average path (VAP, \u03bcm\/s), straight line (VSL, \u03bcm\/s) and curved line velocities (VCL, \u03bcm\/s), straightness, (VSL\/VAP, %) and linearity, (VSL\/VCL, %) as well as supra\u2010vital plasma membrane integrity (SV\u2010PMI, %), mitochondrial membrane potential (MMP, %), viable\/intact acrosome (V\u2013IACR, %) and DNA integrity (DNA\u2013I, %) had significantly greater values (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) during single freeze\u2013thawing as compared with the double freeze\u2013thawing at 0, 30, 90, 150 and 210\u2009days, respectively. All CASA and other assays alone did not show significant differences (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05) between both freeze\u2013thaw cycles at all treatment durations, respectively. No statistical significance (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05) was observed for the in vivo fertility between single (<jats:italic>n<\/jats:italic>\u00a0=\u00a084\/141\u00a0=\u00a059.72%) and double freeze\u2013thawing (<jats:italic>n<\/jats:italic>\u00a0=\u00a072\/136\u00a0=\u00a052.9%) cycles, respectively. In conclusion, sperm motion, kinematics, plasma membrane, acrosome, mitochondria and DNA integrities and in vivo fertility are acceptable after the double freezing protocol despite being lower than after one freeze cycle in Beetal buck.<\/jats:p>","DOI":"10.1111\/rda.14238","type":"journal-article","created":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T09:49:34Z","timestamp":1661507374000},"page":"1615-1622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Validation of double freezing protocol for Beetal buck (<i>Capra hircus<\/i>) spermatozoa"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3766-8800","authenticated-orcid":false,"given":"Syed Aftab Hussain","family":"Shah","sequence":"first","affiliation":[{"name":"Animal Reproduction Laboratory Animal Sciences Institute National Agricultural Research Centre Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Shafiq","family":"Haider","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory Animal Sciences Institute National Agricultural Research Centre Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3180-8208","authenticated-orcid":false,"given":"Hussain","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory Animal Sciences Institute National Agricultural Research Centre Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Hammad","family":"Fayyaz","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory Animal Sciences Institute National Agricultural Research Centre Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syed Murtaza Hassan","family":"Andrabi","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory Animal Sciences Institute National Agricultural Research Centre Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.5194\/aab-61-459-2018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4102\/jsava.v85i1.972"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.05.010"},{"key":"e_1_2_9_5_1","first-page":"159","article-title":"Effect of non\u2010enzymatic antioxidants in extender on post\u2010thaw quality of buffalo (Bubalus bubalis) bull spermatozoa","volume":"28","author":"Andrabi S.","year":"2008","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781780643168.0380"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-8214-7_6"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00700-6"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:2002044"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.06.046"},{"key":"e_1_2_9_11_1","volume-title":"Applied animal reproduction","author":"Bearden H. J. H. J.","year":"2004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep117"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00121-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137495"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1994.tb00748.x"},{"key":"e_1_2_9_16_1","first-page":"23","article-title":"The Effect of Different Dilution Rates of Angora Buck Semen Frozen with Bioxcell\u00ae Extender on the Post\u2010thaw Quality*","volume":"8","author":"Daskin A.","year":"2011","journal-title":"Journal of The Faculty of Veterinary Medicine Erciyes"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.04.005"},{"key":"e_1_2_9_18_1","volume-title":"Semen production and artificial insemination of white\u2010tailed deer","author":"Drew M.","year":"2004"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.17352\/ijvsr.000039"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-35982014001000001"},{"key":"e_1_2_9_21_1","first-page":"17898","article-title":"Cryopreservation of south African indigenous goat semen","volume":"10","author":"Fhulufhelo R.","year":"2011","journal-title":"African Journal of Biotechnology"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.05.012"},{"key":"e_1_2_9_23_1","volume-title":"Pakistan Livestock Census","author":"GOP","year":"2006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.006"},{"key":"e_1_2_9_25_1","volume-title":"Reproduction in farm animals","author":"Hafez E. S. E.","year":"2013"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10228-006-0383-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00239-3"},{"key":"e_1_2_9_28_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.56808\/2985-1130.2727","article-title":"The goat semen quality after being frozen using albumin and cholesterol substituted for egg yolk in semen extender","volume":"46","author":"Kaewkesa T.","year":"2016","journal-title":"The Thai Journal of Veterinary Medicine"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1998.2128"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2009.90286"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299209110020"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2014.04.009"},{"key":"e_1_2_9_33_1","first-page":"39","article-title":"The effect of different glycerol concentrations on freezability of semen from angora, Kilis and Saanen goats","volume":"46","author":"Kulaks\u0131z R.","year":"2013","journal-title":"Slovak Journal of Animal Science"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00156-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2018.0113"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.02.006"},{"key":"e_1_2_9_37_1","first-page":"94","article-title":"The use of the acridine orange test and the TUNEL assay to assess the integrity of freeze\u2010dried bovine spermatozoa DNA","volume":"6","author":"Martins C. F.","year":"2007","journal-title":"Genetics and Molecular Research: GMR"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00811.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.247.3.C125"},{"key":"e_1_2_9_40_1","first-page":"71","volume-title":"General effects of temperature on animal biology","author":"McCue M.","year":"2004"},{"issue":"2","key":"e_1_2_9_41_1","first-page":"157","article-title":"Estrus synchronization and artificial insemination in goats during low breeding season\u2010a preliminary study","volume":"31","author":"Mehmood A.","year":"2011","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12030352"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.5424\/sjar\/2017151-9761"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.06.006"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735782"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01097.x"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022586505089"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2005.02.015"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.08.015"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00155-X"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.11.005"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12214"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(72)85544-9"},{"key":"e_1_2_9_54_1","first-page":"32","article-title":"Effects of Orvus Es Paste (OEP) on the viability of bull spermatozoa after double freezing and thawing","volume":"16","author":"Shimazaki M.","year":"2018","journal-title":"International Journal of Applied Research in Veterinary Medicine"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.06.010"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2016.01.014"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12279"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00664-1"},{"key":"e_1_2_9_59_1","unstructured":"Visconti P.E. Navarrete F. 2019.Method to prepare sperm. Google Patents."},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00099-3"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14238","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.14238","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,26]],"date-time":"2023-11-26T06:09:50Z","timestamp":1700978990000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,5]]},"references-count":59,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1111\/rda.14238"],"URL":"https:\/\/doi.org\/10.1111\/rda.14238","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,5]]},"assertion":[{"value":"2022-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}