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Also, this study aimed to investigate the relationship between temperature\u2010humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI\u2009&lt;68) was higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) than cows receiving a single AI (42.9% vs. 36.4%). P\/AI of cows receiving two AI with severe heat stress (THI\u2009&gt;85) was higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) P\/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed\u2010sorted semen, P\/AI to timed AI was higher (65.0 vs. 51.9%;<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.<\/jats:p>","DOI":"10.1111\/rda.14250","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T07:34:08Z","timestamp":1662622448000},"page":"39-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Fertility of Holstein cows and heifers submitted to timed artificial insemination and receiving one or two doses (12 h apart) of semen"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3341-0060","authenticated-orcid":false,"given":"Miguel","family":"Mellado","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition Autonomous Agrarian University Antonio Narro Saltillo 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