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The detected oligomycin\u2010sensitive basal respiration supported ATP production; frozen\u2013thawed spermatozoa were found to have a coupling efficiency higher than 0.80. Cell respiration, however, was not stimulated by the protonophoric action of FCCP, as its titration with 1, 2, 4 and 6\u00a0\u03bcM did not stimulate the uncoupling activity on oxidative phosphorylation as highlighted by unresponsive oxygen consumption. The unusual effect on the stimulation of maximal respiration was not related to fibronectin\u2010 or PDL\u2010coated plates used for cellular metabolism analysis. Conversely, irradiation of frozen\u2013thawed bovine sperm with the red light improved mitochondrial parameters. In effect, the maximal respiration of red\u2010light\u2010stimulated sperm in PDL\u2010coated plates was higher than the non\u2010irradiated. 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