{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T01:25:08Z","timestamp":1752283508715,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T00:00:00Z","timestamp":1666828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pre\u2010loading bovine sperm with cholesterol prior to freezing is known to increase cryosurvival, though the timing of capacitation in these sperm has not been evaluated. The objective of this study was to determine if there is a potential delay in capacitation timing in these sperm due to the increased cholesterol content. Flow cytometric evaluation was utilized to assess viability, and stain technology to assess acrosome intactness (Propidium Iodide\/FITC\u2010PNA), intracellular calcium levels (Propidium Iodide\/FLUO 3\u2010AM) and membrane fluidity (Merocyanine 540\/YO\u2010PRO\u20101). Cholesterol\u2010loaded cyclodextrin (CLC) (2\u00a0mg\/mL) improved post\u2010thaw viability to 61% from 45% in control sperm (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The addition of ionomycin (0.05\u2009mM) induced capacitation in sperm by 1\u2009h, resulting in increased intracellular calcium and increased acrosome reaction, and consequently viability loss by 3\u2009h. Treatment with CLC significantly decreased membrane fluidity in sperm (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, CLC\u2010treated sperm required 1\u2009h more to capacitate when compared with non\u2010treated sperm based on percentage of live cells with high membrane disorder (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Increased cryosurvival and viability over time was observed, but longer time to capacitate may hinder fertilization capacity and\/or require adjustments to timing of in vitro fertilization.<\/jats:p>","DOI":"10.1111\/rda.14277","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T11:42:30Z","timestamp":1665574950000},"page":"176-183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of cholesterol\u2010loaded cyclodextrin treatment of bovine sperm on capacitation timing"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7157-5440","authenticated-orcid":false,"given":"Gerica","family":"LaVelle","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betsy","family":"Cairo","sequence":"additional","affiliation":[{"name":"CryoGam Colorado  Loveland Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer P.","family":"Barfield","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12611"},{"key":"e_1_2_11_3_1","first-page":"64","volume-title":"Evaluation of breeding soundness","author":"Alexander J.","year":"2014"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0737\u20100806(87)80025\u20104"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.06.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/38.2.291"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527608982.ch1"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402650413"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod35.2.413"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.02.007"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD05003"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199410000304"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14202"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2527\/2006.844826x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2009.06.013"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3000665"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304273200"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20109861(92)90084\u2010A"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093\u2010691X(95)00271\u20109"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.044"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.27.16026"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2003.12.001"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.025577"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401980312"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.5.3235"},{"key":"e_1_2_11_26_1","first-page":"189","volume-title":"The effect of low temperature on biological membranes","author":"Watson P. 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