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Semen collection was performed using artificial vagina from eight Beni Arouss bucks. Ejaculates were pooled, divided into 12 equal aliquots, washed and diluted to 400\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>sperm\/ml (with 7% of glycerol). Skim milk\u2013based extender was supplemented with different concentrations of OM EO (0%; 0.01%; 0.02%; 0.03%; 0.04% and 0.05%) without antibiotics, marked as (CTR\u2212), (M1\u2212), (M2\u2212), (M3\u2212), (M4\u2212) and (M5\u2212), and with 50\u2009mg of streptomycin and 50,000\u2009IU of penicillin per 100\u2009ml, marked as (CTR+), (M1+), (M2+), (M3+), (M4+) and (M5+), respectively. Aliquots were cooled to 4\u00b0C, then frozen in 0.25\u2009ml straws with a programmable freezer and finally stored in liquid nitrogen for 48\u2009h. Thawing was performed at 37\u00b0C for 30\u2009s. Motility, live sperm, sperm abnormalities, membrane integrity, lipid peroxidation and bacterial growth were evaluated after thawing. Among the tested extenders, M2+ improved all semen quality parameters. Sperm motility, live sperm and membrane integrity increased significantly, while the number of abnormal sperm and bacterial growth decreased significantly. The toxic effect of OM EO, with and without antibiotics, appeared beyond 0.03%. In conclusion, M2+ is recommended to improve the cryopreservation of Beni Arouss buck semen.<\/jats:p>","DOI":"10.1111\/rda.14285","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T13:55:05Z","timestamp":1666360505000},"page":"288-297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of<i>Origanum majorana<\/i>essential oil and antibiotics on the quality of frozen thawed Beni Arouss buck semen"],"prefix":"10.1111","volume":"58","author":[{"given":"Amr","family":"Kchikich","sequence":"first","affiliation":[{"name":"Department of Biology Abdelmalek Essaadi University Tangier Morocco"},{"name":"Regional Center of Agricultural Research of Tangier National Institute of Agricultural Research Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Kirschvink","sequence":"additional","affiliation":[{"name":"Department of Medicine, Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6223-6797","authenticated-orcid":false,"given":"Sara","family":"El Kadili","sequence":"additional","affiliation":[{"name":"Department of Animal Production National School of Agriculture of Meknes Meknes Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Raes","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"El Otmani","sequence":"additional","affiliation":[{"name":"Regional Center of Agricultural Research of Tangier National Institute of Agricultural Research Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef","family":"Chebli","sequence":"additional","affiliation":[{"name":"Regional Center of Agricultural Research of Tangier National Institute of Agricultural Research Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Loup","family":"Bister","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bouchra","family":"El Amiri","sequence":"additional","affiliation":[{"name":"Regional Center of Agricultural Research of Settat National Institute of Agricultural Research Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Said","family":"Barrijal","sequence":"additional","affiliation":[{"name":"Department of Biology Abdelmalek Essaadi University Tangier Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6802-9350","authenticated-orcid":false,"given":"Mouad","family":"Chentouf","sequence":"additional","affiliation":[{"name":"Regional Center of Agricultural Research of Tangier National Institute of Agricultural Research Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0094\u20100143(02)00081\u20102"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.11.009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113318"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015\u20100282(97)00241\u20100"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.11.017"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.07.018"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.06.011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102605.1996.tb00475.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1401876111"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.12.007"},{"key":"e_1_2_10_12_1","volume-title":"Salamon's artificial insemination of sheep and goats","author":"Evans G.","year":"1987"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/692751"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2016.1162816"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010021\u201003002\u20109"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.03.003"},{"key":"e_1_2_10_17_1","first-page":"139","volume-title":"Alimentation des bovins, ovins et caprins. 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