{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:55:48Z","timestamp":1771300548734,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objectives of the study were to determine (1) oestrous expression rate and (2) the effect of oestrous expression prior to progesterone\u2010based Ovsynch protocol on pregnancy rate in Holstein cows. All cows (<jats:italic>n<\/jats:italic>\u00a0=\u00a0917) were subjected to 7\u2010day progesterone\u2010based Ovsynch protocol. In this protocol, cows that expressed oestrus before (HEAT1) the scheduled second GnRH were inseminated 20\u2009h later after the onset of oestrus without GnRH administration. Cows that expressed oestrus after the second GnRH administration (HEAT2) or did not express oestrus (NOHEAT) received fixed\u2010timed AI. Oestrous expression was determined by using activity\u2010rumination monitoring system and all cows were inseminated with sexed semen. Oestrous expression rate prior to FTAI was 40.5% and the majority (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.01) of oestrous expression were in HEAT2 compared with HEAT1 in both primiparous (71.8 vs. 28.1%) and multiparous cows (69.5 vs. 30.5%). The mean interval from intravaginal device removal to the onset of oestrus was 47.4\u00a0\u00b1\u20090.9\u00a0h and 62.9\u00a0\u00b1\u20090.5 in HEAT1 and HEAT2, respectively. Primiparous cows (47.7%) had a higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.01) expression rate compared with multiparous cows (37.2%). Overall pregnancy rate was 37.4% and there was two\u2010way significant interaction between parity and oestrous expression on pregnancy rate (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.01). Both primiparous (48.1 vs. 35.8%) and multiparous cows (47.4 vs. 28.4%) that expressed oestrus had greater (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.01) pregnancy rate compared with cows that did not express oestrus. There was no difference in pregnancy rates of HEAT1 and HEAT2 in both primiparous (44.7 vs. 49.5%) and multiparous cows (47.2 vs. 47.6%). Pregnancy rate was not influenced (<jats:italic>p\u2009=<\/jats:italic>\u00a0.21) by milk production (high or low) in both primiparous (47.6 vs. 48.6%) and multiparous (54.9 vs. 42.1%) cows that expressed oestrus, respectively. In conclusion, cows showing oestrus before or after second GnRH of the Ovsynch protocol had greater pregnancy rate than cows not showing oestrus.<\/jats:p>","DOI":"10.1111\/rda.14293","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T09:44:44Z","timestamp":1667987084000},"page":"342-348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of oestrous expression prior to timed artificial insemination with sexed semen on pregnancy rate in dairy cows"],"prefix":"10.1111","volume":"58","author":[{"given":"Baris","family":"Guner","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Bal\u0131kesir University  Bal\u0131kesir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melih","family":"Erturk","sequence":"additional","affiliation":[{"name":"Uluova Dairy Farm  Canakkale Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet","family":"Dursun","sequence":"additional","affiliation":[{"name":"Uluova Dairy Farm  Canakkale Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buse","family":"Ozturk","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Bal\u0131kesir University  Bal\u0131kesir Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gulnaz","family":"Yilmazbas\u2010Mecitoglu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Bursa Uludag University  Bursa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulkadir","family":"Keskin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Bursa Uludag University  Bursa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serdal","family":"Dikmen","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Medicine Bursa Uludag University  Bursa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3607-4365","authenticated-orcid":false,"given":"Ahmet","family":"Gumen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Bursa Uludag University  Bursa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"issue":"3","key":"e_1_2_10_2_1","first-page":"139","article-title":"History, evolution and perspectives of timed artificial insemination programs in Brazil","volume":"9","author":"Baruselli P. 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