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This study aimed to (i) evaluate if hCG administered by the intramuscular (i.m.) or intravaginal (i.vag.) route can be detected by a rapid \u03b2\u2010hCG test in blood plasma samples and (ii) document ovarian effects of hCG administered by both routes at the time of artificial insemination (AI) performed 60\u2009h after oestrus synchronization in goats. Twenty\u2010two Alpine goats received two i.m. injections of 30\u2009\u03bcg of d\u2010cloprostenol (Prolise\u00ae, Tecnopec, S\u00e3o Paulo, Brazil) 7.5\u2009days apart. One day after the onset of oestrus (at the time of AI), the goats were randomly allocated to one of the three groups that received: control (<jats:italic>n<\/jats:italic>\u00a0=\u00a07): 0.3\u00a0ml of saline solution intravaginally; hCG<jats:sub>i.m.<\/jats:sub> (<jats:italic>n<\/jats:italic>\u00a0=\u00a07): 300\u2009IU of hCG (Vetecor\u00ae; Hertape\u2010Calier, S\u00e3o Paulo, Brazil) i.m. and hCG<jats:sub>i.vag.<\/jats:sub> (<jats:italic>n<\/jats:italic>\u00a0=\u00a08): 300\u2009IU of hCG deposited intravaginally. Blood samples were drawn at \u22121, 3, 6, 9 and 24\u2009h after as well as on days 3, 7, 10, 13, 17 and 21 after hCG treatment\/AI. All animals tested negative for hCG (ECO Diagn\u00f3stica, Corinto, Brazil) at \u22121\u00a0h, and all control animals tested negative throughout the entire blood collection period. All hCG<jats:sub>i.m.<\/jats:sub> animals tested positive from 3\u00a0h until D3 post\u2010AI but only 50% of hCG<jats:sub>i.vag.<\/jats:sub> goats tested positive during the present study. In all animals studied, mean circulating P<jats:sub>4<\/jats:sub> concentrations increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) from D3 to D7 after AI and then declined (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) from D10 to D17 in control and hCG<jats:sub>i.m.<\/jats:sub> groups and from D17 to D21 in the hCG<jats:sub>i.vag.<\/jats:sub> group. Total cross\u2010sectional luteal area (CLA), mean colour Doppler area (DA), DA\/CLA, mean high\u2010velocity Doppler area and HVDA\/CLA all declined (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) by D17\u2013D21 in all animals studied. In summary: (i) human chorionic gonadotropin could consistently be detected in blood samples using the rapid \u03b2\u2010hCG test only in the hCG<jats:sub>i.m.<\/jats:sub> group; and (ii) there were no significant differences in the mean pregnancy rate, circulating P<jats:sub>4<\/jats:sub> concentrations and various luteal parameters studied among Control, hCG<jats:sub>i.m.<\/jats:sub> and hCG<jats:sub>i.vag.<\/jats:sub> dose.<\/jats:p>","DOI":"10.1111\/rda.14298","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T14:09:40Z","timestamp":1669730980000},"page":"396-404","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Luteal function in cyclic goats treated with human chorionic gonadotropin administered by intramuscular or intravaginal routes at the time of artificial insemination"],"prefix":"10.1111","volume":"58","author":[{"given":"Juliana Nascimento Duarte","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Domingos","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo S\u00e9rgio Cerqueira","family":"Rangel","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7730-290X","authenticated-orcid":false,"given":"Maria Emilia Franco","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista  Jaboticabal Brazil"},{"name":"Embrapa Caprinos e Ovinos  Coronel Pacheco Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-5562","authenticated-orcid":false,"given":"Felipe Zandonadi","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria Universidade Federal Fluminense  Niter\u00f3i Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-9137","authenticated-orcid":false,"given":"Pawel Mieczyslaw","family":"Bartlewski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8028-3984","authenticated-orcid":false,"given":"Jeferson Ferreira","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Embrapa Caprinos e Ovinos  Coronel Pacheco Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104320(03)00158\u20101"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010018\u20101696\u20102"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2019.106172"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.1997.tb48549.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106571"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3923\/javaa.2012.2091.2096"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015\u20100282(97)00545\u20101"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2020.106579"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.21451\/1809\u20103000.RBRA2021.040"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/BF03548217"},{"key":"e_1_2_9_12_1","volume-title":"Programa de Melhoramento Gen\u00e9tico de Caprinos Leiteiros\u2014Capragene, 3o Sum\u00e1rio de Avalia\u00e7\u00e3o Gen\u00e9tica, 2o Sum\u00e1rio de Avalia\u00e7\u00e3o Gen\u00e9tica Gen\u00f4mica, Ano 2020\u2014Ra\u00e7a Saanen","author":"Faco O.","year":"2020"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984\u20103143\u2010AR2017\u2010957"},{"issue":"4","key":"e_1_2_9_14_1","first-page":"410","article-title":"Effects of hCG on progesterone concentrations and fertility in cyclic, lactating Alpine goats","volume":"3","author":"Fonseca J. 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