{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T05:40:11Z","timestamp":1784698811117,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:00:00Z","timestamp":1671494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of microfluidic technology is increasing in artificial reproduction technologies: With a small amount of semen, it allows for the selection of sperm with the best characteristics of kinetics, morphology and chromatin integrity. The ZyMot Multi (850\u2009\u03bcl) is the most popular device of ZyMot Fertility Inc. To date, it was proven to be a valid instrument for sperm selection for in vitro fertilization and intracytoplasmic sperm injection in men. The aim of this study was to test the efficacy of the ZyMot Multi (850\u2009\u03bcl) for stallion semen. Frozen\u2013thawed semen from 15 stallions that were previously classified as being of \u2018good fertility\u2019 (GF; <jats:italic>n<\/jats:italic>\u00a0=\u00a08; pregnancy rate\u2009\u2265\u200940%) and \u2018poor fertility\u2019 (PF; <jats:italic>n<\/jats:italic>\u00a0=\u00a07; pregnancy rate\u2009&lt;\u200920%), respectively, was used. Each ejaculate was assessed before and after microfluid recovery for kinetics (CASA), membrane integrity (MI) (SYBR14\/PI), membrane functionality (MF) (HOS test), acrosome integrity (Spermac Stain Kit), morphology (Spermac stain kit), mitochondrial membrane potential (MMP) (JC\u20101) and chromatin integrity (aniline blue staining). Sperm concentration was reduced after sperm recovery in both groups, but more markedly in frozen\u2013thawed semen of PF stallions (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Microfluid recovery increased total motility, MI, MF and MMP. While there was a significant increase in the percentage of progressively motile sperm after sperm microfluid recovery, there was a decrease in DAP, DSL, VAP, VSL, LIN, WOB and ALH (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). A slight increase (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) was detected in beat\u2010cross frequency. The present results suggest that the ZyMot Multi (850\u2009\u03bcl) device selects a specific sperm population from any stallion ejaculate with motile sperm and could therefore be a valid tool for in vitro testing with the aim to predict the fertility of frozen\u2013thawed stallion semen.<\/jats:p>","DOI":"10.1111\/rda.14305","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T08:05:00Z","timestamp":1670918700000},"page":"443-449","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Selection of frozen\u2013thawed stallion semen by microfluidic technology"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0357-2579","authenticated-orcid":false,"given":"Veronica","family":"Vigolo","sequence":"first","affiliation":[{"name":"Department for Small Animals and Horses Artificial Insemination and Embryo Transfer, Vetmeduni Vienna  Vienna Austria"},{"name":"Department of Animal Medicine, Production and Health (MAPS) Universit\u00e0 di Padova  Legnaro (PD) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Gautier","sequence":"additional","affiliation":[{"name":"Department for Small Animals and Horses Artificial Insemination and Embryo Transfer, Vetmeduni Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Falomo","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Production and Health (MAPS) Universit\u00e0 di Padova  Legnaro (PD) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6077-7362","authenticated-orcid":false,"given":"Christine","family":"Aurich","sequence":"additional","affiliation":[{"name":"Department for Small Animals and Horses Artificial Insemination and Embryo Transfer, Vetmeduni Vienna  Vienna 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