{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:09Z","timestamp":1787016129433,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002753","name":"Universidad Nacional de Colombia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002753","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010459","name":"Fundaci\u00f3n para el futuro de Colombia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010459","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cryopreservation of semen is an important technique to preserve genetic material. Yet, pregnancy rates in jennies after artificial insemination with frozen\u2013thawed donkey semen are poor. This condition has been attributed to the impact of permeable cryoprotectants, that could cause high post\u2010breeding endometritis. Removal of seminal plasma (SP) prior to semen freezing process is another contributing factor. SP is involved in a multitude of sperm functions and events preceding fertilization and has a mediating effect of sperm capacitation and postcoital uterine inflammatory response. The aim of this study was to evaluate different alternatives in donkey semen cryopreservation with permeable, non\u2010permeable cryoprotectants, BSA and SP. Thirty ejaculates from 10 donkeys were cryopreserved with different combinations of dimethylformamide (DMF, 5%), sucrose (SUC, 200\u2009mM) and homologous SP (10%): DMF (T1), DMF\/SP (T2), SUC\/BSA (T3), SUC\/BSA\/SP (T4), DMF\/SUC\/BSA (T5), DMF\/SUC\/BSA\/SP (T6), DMF\/BSA (T7) and DMF\/BSA\/SP (T8). After thawing, sperm motility and kinetics were assessed by computerized semen analysis. Sperm vitality (SV) was evaluated by fluorescence microscopy, functional membrane integrity (FMI) by the HOST test, abnormal morphology by eosin\u2010nigrosin staining and sperm membrane stability by flow cytometry. For statistical analysis, sperm quality indexes (SQi) were obtained, general linear models were carried out and mean comparisons were made by the Tukey test. T1, T2, T5, T6, and T7 had higher and equivalent results for motility, most kinetic parameters and function membrane integrity. Cryopreservation of donkey semen without permeable cryoprotectant (T3 and T4) showed a reduction in motility, kinetics, SV, FMI and SQi. T5 showed a reduction in progressive motility, sperm velocities, IMF and SQi compared to other DMF treatments. T6 and T8 achieved higher SQi values compared to T1, but they were not different compared to T2 and T7. T1 had a smaller sperm population with low\u2010M540 compared to T3. It is concluded that the use of permeable cryoprotectant is essential to achieve higher post\u2010thaw quality of donkey semen. In addition, the combined use of BSA, SUC and\/or PS may provide additional sperm protection compared to the individual use of DMF.<\/jats:p>","DOI":"10.1111\/rda.14309","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T08:34:15Z","timestamp":1672389255000},"page":"486-495","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Donkey semen cryopreservation: Alternatives with permeable, non\u2010permeable cryoprotectants and seminal plasma"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2272-8185","authenticated-orcid":false,"given":"Juan David","family":"Montoya\u00a0P\u00e1ez","sequence":"first","affiliation":[{"name":"Faculty of Agricultural Sciences, Polit\u00e9cnico Colombiano Jaime Isaza Cadavid  Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0537-0065","authenticated-orcid":false,"given":"Alexandra","family":"\u00dasuga\u00a0Suarez","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science, Universidad CES  Medellin Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8427-4433","authenticated-orcid":false,"given":"Giovanni","family":"Restrepo\u00a0Betancur","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Sciences Universidad Nacional de Colombia  Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD14449"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP.1.00096"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANIREPROSCI.2005.07.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/J.1939\u20104640.2001.TB03446.X"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10561\u2010008\u20109081\u20104"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANIREPROSCI.2017.12.021"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(03)00174\u20102"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.04.007"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JEVS.2017.11.008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.11.005"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","unstructured":"Consuegra C. 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