{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:10:33Z","timestamp":1793261433415,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005680","name":"Gauteng Department of Agriculture and Rural Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005680","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007537","name":"Agricultural Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objectives of this study were to compare oestrous synchronization expression and conception rate following timed artificial insemination (TAI) with frozen\u2013thawed X\u2010sexed or unsexed semen in dairy and beef cows. For this study, 227 cows (dairy, <jats:italic>n<\/jats:italic>\u00a0=\u2009130 and beef, <jats:italic>n<\/jats:italic>\u00a0=\u200997) were assigned to a 9\u2010day Ovsynch\u2009+\u2009controlled intravaginal drug release (CIDR) protocol. All cows were TAI using X\u2010sexed or unsexed semen from 8\u00a0sires. Each semen type was obtained from 4 sires [2 dairy (Holstein Friesian) and 2 beef (Angus)]. Pregnancy detection was performed on Days 35, 65 and 95 following TAI by transrectal ultrasonography and hand palpation. The proportion of oestrus expression was higher in dairy (85.3%) cows compared with beef (65.0%) cows (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). Overall, dairy (X\u2010sexed, 61.9% and unsexed, 62.0%) cows had greater conception rates on Day 35 compared to beef (X\u2010sexed, 56.0% and unsexed, 52.2%) cows (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Concurrently, on Day 95, overall conception rates in dairy (X\u2010sexed, 41.4% and unsexed, 48.5%) cows were greater than beef (X\u2010sexed, 38.0% and unsexed, 37.0%) cows (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). Pregnancy\/embryo losses between Days 35 and 65 in dairy (X\u2010sexed, 33.3% and unsexed, 18.2%) cows and beef (X\u2010sexed, 28.6% and unsexed, 29.2%) cows were recorded (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). Dairy (X\u2010sexed, 7.7% and unsexed, 8.3%) cows had higher incidence of pregnancy losses between Days 66 and 95 when compared to beef (X\u2010sexed, 5.0% and unsexed, 0.0%) cows (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). Oestrous expression and conception rates in dairy and beef cows were satisfactory. Advanced reproductive biotechnologies can successfully utilize gender\u2010ablated semen in organized emerging cattle farming systems.<\/jats:p>","DOI":"10.1111\/rda.14323","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T17:46:10Z","timestamp":1673891170000},"page":"529-536","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Application of gender\u2010ablated semen during timed artificial insemination following oestrous synchronization in dairy and beef cows"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1258-4085","authenticated-orcid":false,"given":"Thabang Luther","family":"Magopa","sequence":"first","affiliation":[{"name":"Germplasm Conservation and Reproduction Biotechnologies, Agricultural Research Council Animal Production  Pretoria South Africa"},{"name":"Department of Agriculture and Animal Health University of South Africa  Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2350-9593","authenticated-orcid":false,"given":"Masindi Lottus","family":"Mphaphathi","sequence":"additional","affiliation":[{"name":"Germplasm Conservation and Reproduction Biotechnologies, Agricultural Research Council Animal Production  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6030-3068","authenticated-orcid":false,"given":"Thendo","family":"Mulaudzi","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Animal Health University of South Africa  Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00625.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.09.021"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.04.011"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731114000664"},{"key":"e_1_2_10_6_1","unstructured":"Carwell D. B.(2010).Pregnancy rates in beef cattle artificially inseminated with frozen\u2010thawed aged beef semen. Master's Thesis La. State University Baton Rouge."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2006-694"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2301"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.01.003"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984-3143-AR2018-0070"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011-4214"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0964"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0720(15)30647-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.05.021"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.09.025"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.009"},{"issue":"11","key":"e_1_2_10_17_1","first-page":"283","article-title":"Comparative efficacy of two different synchronization protocol in postpartum dairy cows","volume":"8","author":"Hirole P. 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