{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:47:20Z","timestamp":1790664440140,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,5]],"date-time":"2023-03-05T00:00:00Z","timestamp":1677974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to investigate the effect of\n                    <jats:italic>Spirulina platensis<\/jats:italic>\n                    (SP) and\n                    <jats:italic>Salvia verbenaca<\/jats:italic>\n                    (SV) extracts added to skimmed milk (SM) extender on ram sperm quality and fertility. Semen was collected using an artificial vagina, extended in SM to reach a final concentration of 0.8\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    spermatozoa\/mL, stored at 4\u00b0C and evaluated at 0, 5\u00a0H and 24\u2009H. The experiment has been performed in three steps. Firstly, from four extracts (methanol: MeOH, acetone: Ac, ethyl acetate: EtOAc and hexane: Hex) of SP and SV, only acetonic and hexanoic extracts of SP and acetonic and methanolic extracts of SV showed the highest\n                    <jats:italic>in vitro<\/jats:italic>\n                    antioxidant activities and were then selected for the following step. Thereafter, the effects of four concentrations (1.25, 3.75, 6.25, and 8.75\u2009\u03bcg\/mL) of each selected extract on stored sperm motility were evaluated. The output of this trial led to select the best concentrations having beneficial effects on sperm quality parameters (viability, abnormalities, membrane integrity, and lipid peroxidation) and fertility after insemination. The results showed that the same concentration (1.25\u2009\u03bcg\/mL) of both Ac\u2010SP and Hex\u2010SP, as well as 3.75\u2009\u03bcg\/mL of Ac\u2010SV and 6.25\u2009\u03bcg\/mL of MeOH\u2010SV, maintain all sperm quality parameters at 4\u00b0C during 24\u2009H of storage. Besides, no difference was found in fertility between the selected extracts and the control. In conclusion, SP and SV extracts were shown to improve the quality of ram sperm and to maintain fertility rate after insemination as similar or competitive to many previous studies published in the field.\n                  <\/jats:p>","DOI":"10.1111\/rda.14332","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T03:29:14Z","timestamp":1676863754000},"page":"637-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Inclusion of\n                    <i>Spirulina platensis<\/i>\n                    and\n                    <i>Salvia verbenaca<\/i>\n                    extracts to boost semen quality and fertilization ability in sheep"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2878-2377","authenticated-orcid":false,"given":"Anass","family":"Ben\u00a0Moula","sequence":"first","affiliation":[{"name":"Animal Production Unit, Regional Center Agricultural Research of Settat National Institute for Agricultural Research (INRA)  Settat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdellatif","family":"Rahim","sequence":"additional","affiliation":[{"name":"Animal Production Unit, Regional Center Agricultural Research of Settat National Institute for Agricultural Research (INRA)  Settat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6802-9350","authenticated-orcid":false,"given":"Mouad","family":"Chentouf","sequence":"additional","affiliation":[{"name":"Animal Production Unit, Regional Center for Agricultural Research of Tangier National Institute for Agricultural Research (INRA)  Tangier Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Raes","sequence":"additional","affiliation":[{"name":"Integrated Veterinary Research Unit, Narilis University of Namur  Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Kirschvink","sequence":"additional","affiliation":[{"name":"Unit of molecular physiology, Faculty of Medicine, Narilis University of Namur  Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bouchra","family":"El\u00a0Amiri","sequence":"additional","affiliation":[{"name":"Animal Production Unit, Regional Center Agricultural Research of Settat National Institute for Agricultural Research (INRA)  Settat Morocco"},{"name":"African Sustainable Agriculture Research Institute (ASARI) Mohammed VI Polytechnic University (UM6P)  Laayoune Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.112.016535"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/tas\/txaa060"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4236\/abb.2019.104006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.03.019"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.09.013"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1556\/004.2019.043"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.21010\/ajtcam.v14i2.29"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/jcim\u20102019\u20100036"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14304"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/1811199a0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu6052115"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1052"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2010.03.025"},{"issue":"2","key":"e_1_2_10_15_1","first-page":"91","article-title":"Conservation de la semence ovine","volume":"22","author":"Druart X.","year":"2009","journal-title":"NRAE Productions Animales"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40502\u2010014\u20100094\u20107"},{"key":"e_1_2_10_17_1","first-page":"8","volume-title":"Salamon's artificial insemination of sheep and goats","author":"Evans G.","year":"1987"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.06.047"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13273\u2010017\u20100015\u20108"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02659771"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934457"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nexus.2022.100070"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2014.09.016"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11050637"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13472"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.10.006"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.14278"},{"issue":"9","key":"e_1_2_10_28_1","first-page":"331","article-title":"Antioxidant and the immunomodulatory activities exhibited by three plants from Lamiaceae family","volume":"7","author":"Nassar M.","year":"2015","journal-title":"International Journal of Pharmacy and Pharmaceutical Sciences"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.150.10.299"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(66)90167\u20109"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2021.05.006"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0891\u20105849(98)00315\u20103"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.152.25.765"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113940"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12030244"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10050772"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2016.07.010"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7378379"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8178294"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2013.07.006"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.01.004"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13861"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14332","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.14332","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T04:48:50Z","timestamp":1692420530000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,5]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/rda.14332"],"URL":"https:\/\/doi.org\/10.1111\/rda.14332","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,5]]},"assertion":[{"value":"2022-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}