{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:41:12Z","timestamp":1788374472742,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972574"],"award-info":[{"award-number":["31972574"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012579","name":"Natural Science Foundation of Qinghai","doi-asserted-by":"publisher","award":["2019\u2010ZJ\u2010902"],"award-info":[{"award-number":["2019\u2010ZJ\u2010902"]}],"id":[{"id":"10.13039\/501100012579","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2021432"],"award-info":[{"award-number":["2021432"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cattle\u2010yak, the hybrid offspring of yak (<jats:italic>Bos grunniens<\/jats:italic>) and cattle (<jats:italic>Bos taurus<\/jats:italic>), serves as a unique model to dissect the molecular mechanisms underlying reproductive isolation. While female cattle\u2010yaks are fertile, the males are completely sterile due to spermatogenic arrest at the meiosis stage and massive germ cell apoptosis. Interestingly, meiotic defects are partially rescued in the testes of backcrossed offspring. The genetic basis of meiotic defects in male cattle\u2010yak remains unclear. Structure\u2010specific endonuclease subunit (SLX4) participates in meiotic double\u2010strand break (DSB) formation in mice, and its deletion results in defects in spermatogenesis. In the present study, we examined the expression patterns of SLX4 in the testes of yak, cattle\u2010yak, and backcrossed offspring to investigate its potential roles in hybrid sterility. The results showed that the relative abundances of SLX4 mRNA and protein were significantly reduced in the testis of cattle\u2010yak. The results of immunohistochemistry revealed that SLX4 was predominately expressed in spermatogonia and spermatocytes. Chromosome spreading experiments showed that SLX4 was significantly decreased in the pachytene spermatocytes of cattle\u2010yak compared with yak and backcrossed offspring. These findings suggest that SLX4 expression was dysregulated in the testis of cattle\u2010yak, potentially resulting in the failure of crossover formation and collapses of meiosis in hybrid males.<\/jats:p>","DOI":"10.1111\/rda.14338","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T12:45:35Z","timestamp":1678193135000},"page":"679-687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Localization and expression of <scp>SLX4<\/scp> in the testis of sterile male cattle\u2010yak"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9782-5006","authenticated-orcid":false,"given":"Shang\u2010Shang","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Animal Science Xinjiang Agricultural University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010Xin","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology, Chinese Academy of Sciences  Xining China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong\u2010Xue","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology, Chinese Academy of Sciences  Xining China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"Qinghai Key Laboratory of Animal Ecological Genomics Northwest Institute of Plateau Biology, Chinese Academy of Sciences  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wusiman","family":"Abulizi","sequence":"additional","affiliation":[{"name":"College of Animal Science Xinjiang Agricultural University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi\u2010En","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology, Chinese Academy of Sciences  Xining China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"Qinghai Key Laboratory of Animal Ecological Genomics Northwest Institute of Plateau Biology, Chinese Academy of Sciences  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18270"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.00135\u201007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201032164\u20109"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002094"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1276"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/0300\u20105127:0290655"},{"key":"e_1_2_9_8_1","first-page":"23","article-title":"Comparative histologic studies on testes in yak and first to third filial generation by yak and cattle","volume":"10","author":"Jia R. 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