{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:41:13Z","timestamp":1788374473441,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the distribution and expression of matrix metalloproteinase\u20102 (MMP\u20102) and tissue inhibitor of matrix metalloproteinase\u20102 (TIMP\u20102) in yak testes. The testes of healthy yaks at different ages: newborn [3\u2009days], young [1 year], adult [4\u2009years], and old [9\u2009years] were collected for microscopic analyses using hematoxylin and eosin staining, immunohistochemistry and immunofluorescence, as well as western blot to compare the expression of MMP\u20102 and TIMP\u20102. Furthermore, the levels of MMP\u20102<jats:italic>mRNA<\/jats:italic> and TIMP\u20102<jats:italic>mRNA<\/jats:italic> was detected by real\u2010time quantitative polymerase chain reaction (qPCR). The results of immunohistochemistry and immunofluorescence demonstrated that MMP\u20102 and TIMP\u20102 were mainly located in gonocytes of newborn, Sertoli cells of young, spermatozoa of adult and Leydig cells of old. The protein levels of MMP\u20102 and TIMP\u20102 exhibited a downward from newborn to adult, but increased again in old yaks. The analysis of qPCR showed that MMP\u20102 was higher in young compared with newborn or adult(**<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), but a lower expression was detected in adult compared with old yak testicular tissues (*<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Compared with adults, TIMP\u20102 was significantly higher in newborn and young yaks (**<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), and slightly higher in old yaks (*<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Hence, The location of MMP\u20102 and TIMP\u20102 in gonocytes were associated with the development of newborn yak testes. The expression of MMP\u20102 and TIMP\u20102 in Sertoli cells at young and adult yaks suggested that they provided a clue for the regulation of spermatogenesis. The positive labeling of MMP\u20102 and TIMP\u20102 in Leydig cells in old yaks suggested that both may be involved in the interstitial metabolism of the testes during this period. This study revealed the possible role of MMP\u20102 and TIMP\u20102 in testicular functionality of yaks at different ages.<\/jats:p>","DOI":"10.1111\/rda.14345","type":"journal-article","created":{"date-parts":[[2023,3,18]],"date-time":"2023-03-18T05:31:05Z","timestamp":1679117465000},"page":"746-753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of <scp>MMP<\/scp>\u20102 and <scp>TIMP<\/scp>\u20102 expressions in yak testes at different ages"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3720-2741","authenticated-orcid":false,"given":"Juanjuan","family":"Song","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Gansu Agricultural University  Lanzhou China"},{"name":"Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation of Gansu Province  Lanzhou 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