{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T19:54:13Z","timestamp":1783972453372,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T00:00:00Z","timestamp":1681257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010803","name":"Department of Biotechnology, Government of West Bengal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As onset of sepsis adversely affects the prognosis of canine pyometra, finding biomarkers that would distinguish sepsis status would be useful in the clinical management. Accordingly, we hypothesized that differential expression of endometrial transcripts and circulating concentration of certain inflammatory mediators would discriminate pyometra\u2010led sepsis (P\u2010sepsis+) from those of pyometra without sepsis (P\u2010sepsis\u2212). Bitches with pyometra (<jats:italic>n<\/jats:italic>\u2009=\u200952) were classified into P\u2010sepsis+ (<jats:italic>n<\/jats:italic>\u2009=\u200928) and P\u2010sepsis\u2212 (<jats:italic>n<\/jats:italic>\u2009=\u200924) based on vital clinical score and total leukocyte count. A group of non\u2010pyometra bitches (<jats:italic>n<\/jats:italic>\u2009=\u200912) served as control. The relative fold changes in the transcripts of IL6, IL8, TNF\u03b1, IL10, PTGS2, mPGES1 and PGFS, SLPI, S100A8, S100A12 and eNOS were determined by quantitative polymerase chain reaction. Furthermore, the serum\u00a0concentrations of IL6, IL8, IL10, SLPI and prostaglandin F<jats:sub>2\u03b1<\/jats:sub> metabolite (PGFM) were assayed by ELISA. The relative fold changes in S100A12 and SLPI and mean concentrations of IL6 and SLPI were significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) higher in P\u2010sepsis+ than that of P\u2010sepsis\u2212 group. Receiver operating characteristic analysis revealed that serum IL6 had a diagnostic sensitivity of 78.6% and a positive likelihood ratio (LR+) of 2.09, at a cut\u2010off value of 15.7\u2009pg\/mL to diagnose P\u2010sepsis+ cases. Similarly, serum SLPI had a sensitivity of 84.6% and an LR+ of 2.23, at a cut\u2010off value of 2.0\u2009pg\/mL. It was concluded that SLPI and IL6 would serve as putative biomarkers for pyometra\u2010led sepsis in bitches. Monitoring SLPI and IL6\u00a0would be a useful adjunct to the established haemato\u2010biochemical parameters in customizing the treatment strategies and arriving at the decision for management of pyometra bitches\u00a0with critical illness.<\/jats:p>","DOI":"10.1111\/rda.14353","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T15:02:27Z","timestamp":1680534147000},"page":"802-812","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serum concentrations of secretory leukocyte protease inhibitor and <scp>IL6<\/scp> can predict the onset of sepsis in pyometra bitches"],"prefix":"10.1111","volume":"58","author":[{"given":"Jisna Kuttiyeri","family":"Sasidharan","sequence":"first","affiliation":[{"name":"Animal Reproduction Division Indian Council of Agricultural Research (ICAR)\u2010Indian Veterinary Research Institute (IVRI)  Izatnagar 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