{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:34:33Z","timestamp":1792542873944,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T00:00:00Z","timestamp":1682208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the stress\u2010related changes that may occur in sheep following non\u2010surgical cervical line passage. A total of 20 sheep underwent transcervical transition, and their heart rate, respiratory rate, body temperature, and blood samples were collected at specific time points: 1\u2009h prior to the procedure, immediately after the procedure, and at 1, 2, 3, 4 and 5\u2009h post\u2010procedure. The study found that there was a statistically significant decrease in body temperature immediately after the procedure (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05); however, there were no significant differences in body temperature at other time points (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). Respiratory rate remained similar throughout the study, and no significant differences were observed (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). On the other hand, there was a significant increase in heart rate at the 1\u2010h mark after the procedure (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), which subsequently decreased to a similar level as the control group at the 4\u2010h mark after the procedure (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). The cortisol levels increased significantly after the application (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) but decreased to a level similar to the control after 1\u2009h (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). The level of nitric oxide increased significantly in the first hour (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) and decreased by the 5th hour (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). There was a significant increase in malondialdehyde (MDA) and total oxidant capacity (TOC) levels 2\u2009h after the application (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), but this increase ceased at the 4th and 5th hours for MDA (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05) and at the 3rd, 4th and 5th hours for TOC (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). The total antioxidant capacity decreased after the 2nd hour of application (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) but remained similar at the fourth and 5th hours (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). In conclusion, based on the cortisol data, transcervical manipulations create short\u2010term stress. Therefore, it may be necessary to use various antioxidants before such procedures to reduce the effects of oxidative stress.<\/jats:p>","DOI":"10.1111\/rda.14361","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T07:25:50Z","timestamp":1681370750000},"page":"877-881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oxidative response of sheep to transcervical applications"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5141-0008","authenticated-orcid":false,"given":"Nail Tekin","family":"\u00d6nder","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf Umut","family":"Bat\u0131","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taygun","family":"G\u00f6kdemir","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammet Can","family":"K\u0131l\u0131\u00e7","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O\u011fuzhan","family":"\u015eahin","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Metin","family":"\u00d6\u011f\u00fcn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enes","family":"Aky\u00fcz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mushap","family":"Kuru","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Haydar","family":"K\u0131rm\u0131z\u0131g\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sava\u015f","family":"Y\u0131ld\u0131z","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yavuz","family":"\u00d6zt\u00fcrkler","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.12991\/mpj.2014187243"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.03.007"},{"issue":"1","key":"e_1_2_8_4_1","first-page":"1","article-title":"Egzersiz ve stres hormonlar\u0131","volume":"2","author":"Civan A.","year":"2018","journal-title":"T\u00fcrkiye Spor Bilimleri Dergisi"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.12681\/jhvms.15544"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.025"},{"key":"e_1_2_8_7_1","first-page":"312","article-title":"Oxidative stress and total sialic acid levels in sheep naturally infected with pox virus","volume":"36","author":"K\u0131rm\u0131z\u0131g\u00fcl A. 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