{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:54:02Z","timestamp":1790801642686,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T00:00:00Z","timestamp":1684627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007351","name":"Secretaria de Ciencia y Tecnica, Universidad de Buenos Aires","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007351","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to evaluate the effectiveness of the original sperm chromatin dispersion (SCD) assay and the toluidine blue (TB) stain to assess DNA fragmentation and chromatin condensation, respectively, in cat sperm obtained by urethral catheterization (CT) and epididymis slicing (EP). CT and EP samples were collected from the same cat, and sperm motility, concentration, morphology, DNA integrity and chromatin condensation were evaluated. As controls, aliquots of the samples were incubated with 0.3\u2009M NaOH and with 1% of dithiothreitol (DTT) to promote DNA fragmentation and chromatin decondensation, respectively. With SCD, four DNA dispersion halo patterns were observed: large, medium, small and no halo. TB staining patterns were as follows: light blue (condensed chromatin), light violet (moderate chromatin decondensation) and dark blue\u2010violet (high chromatin decondensation). Sperm incubations with NaOH and with DTT were effective in inducing DNA fragmentation and chromatin decondensation, respectively. No significant differences were observed in the percentages of the SCD and TB patterns between samples (CT and EP) and no correlation was observed between sperm head abnormalities and the different SCD and TB patterns. The original SCD technique and the TB stain were adapted to evaluate DNA integrity and chromatin condensation in cat sperm obtained by CT and EP.<\/jats:p>","DOI":"10.1111\/rda.14388","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T14:05:52Z","timestamp":1684245952000},"page":"982-989","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of <scp>DNA<\/scp> integrity and chromatin condensation in cat sperm collected by urethral catheterization and epididymis slicing"],"prefix":"10.1111","volume":"58","author":[{"given":"Cecilia","family":"Allera","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3090-6052","authenticated-orcid":false,"given":"Mar\u00eda Florencia","family":"Gallelli","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah Margarita","family":"Neild","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-0580","authenticated-orcid":false,"given":"Mar\u00eda Ignacia","family":"Carretero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12706"},{"issue":"2","key":"e_1_2_10_3_1","first-page":"85","article-title":"Morphometric features and chromatin condensation abnormalities evaluated by toluidine blue staining in bull spermatozoa","volume":"22","author":"Beletti M. 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