{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:09:09Z","timestamp":1791256149224,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to determine the effect of microfluidic sperm sorting chip on embryo development and quality in the sperm treatment step in in vitro embryo production in cattle. Only A\u2010quality oocytes obtained from the ovaries of Holstein cattle were included in the study. These oocytes were first placed in in vitro maturation medium, and matured oocytes were randomly divided into two groups at the 24th hour of maturation. Oocytes in the first group with the Microfluidic Sperm Sorting Chip (MFSC, <jats:italic>n<\/jats:italic>\u2009=\u2009154) were put into a fertilization medium with spermatozoa prepared with microfluidic sperm sorting device. Oocytes in the second group (Con, <jats:italic>n<\/jats:italic>\u2009=\u2009169) were fertilized with spermatozoa prepared by the routine sperm treatment step of the commercial company. The rate of cleavage (85.71% vs. 76.33%, respectively) and of reaching the blastocyst (44.15% vs. 32.54%, respectively) in the MFSC group were higher than the control group. In addition, it was determined that the numbers of ICM (45.8\u2009\u00b1\u20092.04 vs. 39.2\u2009\u00b1\u20091.85, respectively), TE (122.13\u2009\u00b1\u20092.19 vs. 115.0\u2009\u00b1\u20092.61, respectively), TC (167.93\u2009\u00b1\u20092.89 vs. 154.2\u2009\u00b1\u20092.62, respectively) increased in the MFSC group compared to the control group. A statistically significant difference was found in the number of cells with apoptosis per embryo (5.14\u2009\u00b1\u20090.77 vs. 11.91\u2009\u00b1\u20090.79) and apoptotic index rates (3.06\u2009\u00b1\u20090.47 vs. 7.72\u2009\u00b1\u20090.55%) of the MFSC and Con groups. As a result, we concluded that using microfluidic sperm sorting chips during sperm treatment in bovine IVEP increases the rate of reaching the blastocyst, embryo development, and quality and reduces the possibility of apoptosis in developing blastocysts. For this reason, it is also thought that the use of microfluidic sperm sorting devices during sperm treatment in bovine IVEP may be a new alternative in this field.<\/jats:p>","DOI":"10.1111\/rda.14398","type":"journal-article","created":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T07:40:09Z","timestamp":1685346009000},"page":"1012-1020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Does using microfluidic sperm sorting chips in bovine <scp>IVEP<\/scp> affect blastocyst development?"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8332-5334","authenticated-orcid":false,"given":"Hasan","family":"Alkan","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9877-8405","authenticated-orcid":false,"given":"Fatma","family":"Satilmis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1586-7489","authenticated-orcid":false,"given":"Mehmet Akif","family":"Demirel","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8432-7347","authenticated-orcid":false,"given":"Mustafa","family":"Bodu","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7721-2576","authenticated-orcid":false,"given":"Omer Faruk","family":"Yesilkaya","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-6500","authenticated-orcid":false,"given":"Muhammed Furkan","family":"Ciftci","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1416-5354","authenticated-orcid":false,"given":"Huseyin","family":"Erdem","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4060-7048","authenticated-orcid":false,"given":"Mustafa Agah","family":"Tekindal","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Medicine Izmir Katip \u00c7elebi University  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9177-9299","authenticated-orcid":false,"given":"Kubra Karakas","family":"Alkan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12081010"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","unstructured":"Alagoz O. 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