{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:18:25Z","timestamp":1787015905616,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T00:00:00Z","timestamp":1686268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010803","name":"Department of Biotechnology, Government of West Bengal","doi-asserted-by":"publisher","award":["BT\/PR30439\/AAQ\/1\/743\/2019"],"award-info":[{"award-number":["BT\/PR30439\/AAQ\/1\/743\/2019"]}],"id":[{"id":"10.13039\/501100010803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The use of antibiotics in semen extenders can contribute to the development of antibiotic resistance. The objective of the study was to evaluate epsilon\u2010polylysine (\u0190\u2010PL) as a substitute for antibiotics in the buffalo semen extender. For this, 20 semen ejaculates were collected from four Murrah buffalo bulls. Each ejaculate was divided into three equal aliquots and extended into an egg yolk\u2010based semen extender containing either antibiotics (strepto\u2010penicillin) or different concentrations of \u0190\u2010PL (0.64 and 1.28\u2009g\/L) to make the final concentration 80 million sperm\/mL and cryopreserved as per the standard procedure. The antibiogram sensitivity test confirmed that \u0190\u2010PL is an effective antimicrobial against microbes present in buffalo semen ejaculates. Furthermore, the addition of \u0190\u2010PL in the semen extender significantly reduces the colony forming unit (CFU)\/mL in cryopreserved semen equivalent to strepto\u2010penicillin. The sperm motility and kinematic parameters assessed by a computer\u2010assisted sperm analyser showed that \u0190\u2010PL did not inhibit either sperm motility not kinematic parameters of cryopreserved sperm. The flow\u2010cytometric evaluation of frozen\u2013thawed sperm revealed interesting results. The extender supplemented with \u0190\u2010PL protected sperm acrosome and mitochondrial membrane potential greater than the extender supplemented with strepto\u2010penicillin. Further, \u0190\u2010PL reduced significantly the production of superoxide anions from mitochondria during the cryopreservation process. In this way, \u0190\u2010PL may be a suitable alternative to antibiotics in semen extenders. In conclusion, \u0190\u2010PL at a concentration of 0.64\u2009g\/L acts as an effective antimicrobial as well as antioxidant in semen extender for cryopreservation of buffalo sperm.<\/jats:p>","DOI":"10.1111\/rda.14399","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T02:59:05Z","timestamp":1685501945000},"page":"1070-1079","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Epsilon poly\u2010lysine in buffalo semen extender: A step towards reducing the development of antibiotic resistance"],"prefix":"10.1111","volume":"58","author":[{"given":"Usha","family":"Yadav","sequence":"first","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"},{"name":"Department of Veterinary Gynaecology and Obstetrics Lala Lajpat Rai University of Veterinary and Animal Sciences  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-780X","authenticated-orcid":false,"given":"Ravi","family":"Dutt","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics Lala Lajpat Rai University of Veterinary and Animal Sciences  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna","family":"Bansal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science Navania Rajasthan University of Veterinary and Animal Sciences  Bikaner India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akansha","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Dairy Cattle Physiology ICAR\u2010National Dairy Research Institute Karnal  Karnal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Bala","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Bhardwaj","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Verma","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manju","family":"Bishnoi","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharmendra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tirtha Kumar","family":"Datta","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-2201","authenticated-orcid":false,"given":"Pradeep","family":"Kumar","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14431"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14123"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9080750"},{"issue":"3","key":"e_1_2_10_5_1","first-page":"229","article-title":"Determination of sperm acrosin activity for evaluation of male fertility","volume":"2","author":"Cui Y. 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