{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T22:07:28Z","timestamp":1766441248063,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006514","name":"Federaal Agentschap Voor de Veiligheid Van de Voedselketen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006514","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the presence of <jats:italic>Chlamydia<\/jats:italic> spp. and <jats:italic>Parachlamydia acanthamoebae<\/jats:italic> in bovine placental tissue originating from abortion and non\u2010abortion cases in Belgium. Placentas of 164 late term bovine abortions (last trimester of gestation) and 41 non\u2010abortion (collected after calving) cases were analysed by PCR for <jats:italic>Chlamydia<\/jats:italic> spp., <jats:italic>Chlamydia abortus<\/jats:italic>, <jats:italic>C.\u2009psittaci<\/jats:italic> and <jats:italic>P.\u2009acanthamoebae<\/jats:italic>. Additionally, a subset of 101 (75 abortion and 26 non\u2010abortion cases) of these placenta samples were also analysed by histopathology to detect possible <jats:italic>Chlamydia<\/jats:italic>\u2010induced lesions. In 5.4% (11\/205) of the cases, <jats:italic>Chlamydia<\/jats:italic> spp. were detected, and three of those cases were positive for <jats:italic>C.\u2009psittaci<\/jats:italic>. <jats:italic>Parachlamydia acanthamoebae<\/jats:italic> was detected in 36% (75\/205) of the cases, being 44% (<jats:italic>n<\/jats:italic>\u2009=\u200972) in abortions and 7.3% (<jats:italic>n<\/jats:italic>\u2009=\u20093) in non\u2010abortions cases (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). None of the cases was positive for <jats:italic>C.\u2009abortus<\/jats:italic>. Purulent and\/or necrotizing placentitis with or without vasculitis was observed in 18.8% (19\/101) of the histopathologically analysed placenta samples. In 5.9% (6\/101) of the cases, placentitis was observed along with vasculitis. In the abortion cases, 24% (18\/75) of the samples showed purulent and\/or necrotizing placentitis, while purulent and\/or necrotizing placentitis was visible in 3.9% (1\/26) of the non\u2010abortion cases. Placental lesions of inflammation and\/or necrosis were present in 44% (15\/34) of the cases where <jats:italic>P. acanthamoebae<\/jats:italic> was detected, while inflammation and\/or necrosis was present in 20.9% (14\/67) of the negative cases (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The detection of <jats:italic>Chlamydia<\/jats:italic> spp. and especially <jats:italic>P.\u2009acanthamoebae<\/jats:italic>, in combination with correlated histological lesions such as purulent and\/or necrotizing placentitis and\/or vasculitis in placental tissue following abortion, suggests a potential role of this pathogen in cases of bovine abortion in Belgium. Further in\u2010depth studies are necessary to unravel the role of these species as abortifacient agents in cattle and to include them in bovine abortion monitoring programmes.<\/jats:p>","DOI":"10.1111\/rda.14403","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T14:53:47Z","timestamp":1686236027000},"page":"1080-1086","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of Chlamydia and Chlamydia\u2010like organisms in bovine placental tissue"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3689-1979","authenticated-orcid":false,"given":"Hans","family":"Van\u00a0Loo","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Reproduction, and Population Medicine, Faculty of Veterinary Medicine Ghent University  Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osvaldo Bogado","family":"Pascottini","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction, and Population Medicine, Faculty of Veterinary Medicine Ghent University  Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefaan","family":"Ribbens","sequence":"additional","affiliation":[{"name":"Animal Health Services Flanders  Torhout Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jozef","family":"Hooyberghs","sequence":"additional","affiliation":[{"name":"Federal Agency for the Safety of the Food Chain  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcella","family":"Mori","sequence":"additional","affiliation":[{"name":"Federal Research Institute for Health, Bacterial Zoonoses, Veterinary Bacteriology, Sciensano  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Vanrompay","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology Ghent University  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert","family":"Opsomer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction, and Population Medicine, Faculty of Veterinary Medicine Ghent University  Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"Pardon","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Reproduction, and Population Medicine, Faculty of Veterinary Medicine Ghent University  Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000369"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1469\u20100691.2008.02134.X"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3282f3e6a5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1354\/VP.43\u20105\u2010702"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MCP.2007.06.003"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985817751218\/FORMAT\/EPUB"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818806453"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167\u20105877(01)00197\u20100"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3855\/JIDC.16166"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.4.814\u2010816.1990"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.018"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.6.1512-1518.1996"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/S11259\u2010005\u20100832\u20104\/METRICS"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2051"},{"volume-title":"Histological and histochemical methods: Theory and practice","year":"2008","author":"Kiernan J. 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