{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:53:53Z","timestamp":1790952833575,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of multiple ovulation and embryo transfer (MOET) technology in the dairy cattle industry has increased dramatically in recent decades for the production of offspring from genetically superior cows. Yet, its long\u2010term ramifications on adult performance have not been adequately clarified. Therefore, this study targeted comparing dairy heifers born after the transfer of in vivo\u2010produced embryos (MOET\u2010heifers, <jats:italic>n<\/jats:italic>\u2009=\u2009400) and those born after artificial insemination (AI\u2010heifers, <jats:italic>n<\/jats:italic>\u2009=\u2009340). The performance of MOET\u2010heifers and AI\u2010heifers was compared from birth till completion of the first lactation regarding health, fertility and some lactational performance parameters. The transcript abundance of several genes was also assessed in peripheral blood leukocytes (PBWC). Results showed greater pre\u2010weaning mortalities, greater likelihood of being culled as a nulliparous heifer and younger age at first insemination in AI\u2010heifers (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). At their first calving, primiparous MOET\u2010heifers experienced a greater (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) incidence of stillbirth compared to primiparous AI\u2010heifers. In spite of that, primiparous AI\u2010heifers were more likely to be culled due to infertility (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001), took a greater number of inseminations to achieve pregnancy (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and displayed a longer first calving interval. There was a similar lactational performance between the two groups. Upregulation of the transcript levels of <jats:italic>TAC3, LOC522763, TFF2, SAXO2, CNKSR3<\/jats:italic> and <jats:italic>ALAS2<\/jats:italic> was interestingly observed in primiparous MOET\u2010heifers, compared to primiparous AI\u2010heifers. In conclusion, MOET\u2010heifers were less likely to be culled during the first year of life, had superior reproductive performance versus AI\u2010heifers during their first lactation and expressed upregulation of genes associated with fertility.<\/jats:p>","DOI":"10.1111\/rda.14417","type":"journal-article","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T13:54:34Z","timestamp":1687960474000},"page":"1146-1155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Monitoring of health status, performance and transcript abundance of some genes in dairy heifers produced by embryo transfer or artificial insemination"],"prefix":"10.1111","volume":"58","author":[{"given":"Rabie L.","family":"Abdel\u00a0Aziz","sequence":"first","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud M.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussein","family":"El\u2010Said","sequence":"additional","affiliation":[{"name":"Veterinary and Embryo Transfer Sector Milkys Company  El\u2010Nobaria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaimaa","family":"Kamel","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-8953","authenticated-orcid":false,"given":"Ahmed","family":"Abdel\u2010Wahab","sequence":"additional","affiliation":[{"name":"Physiology Department, Faculty of Veterinary Medicine Minia University  El\u2010Minya Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346\u2010023\u201001301\u20102"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-016-0905-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2006-504"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2018.06.007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-2432"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00091-6"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2858"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(06)72097-5"},{"key":"e_1_2_9_11_1","first-page":"385","article-title":"Fertility in dairy cows subjected to two different intervals from presynchronization to initiation of Ovsynch protocol","volume":"24","author":"Colazo M. 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