{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T16:29:22Z","timestamp":1749313762373,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T00:00:00Z","timestamp":1688601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of \u03b12\u2010adrenergic agonists in association with urethral catheterization has been used as a technique for pharmacological semen collection in cats. The mechanism of action of this drug is the stimulation of adrenoreceptors in the vas deferens, which results in ejaculation. While medetomidine is the \u03b12\u2010agonist most commonly used in studies, ejaculation with the use of dexmedetomidine associated with ketamine has been effective, but with variable results. Therefore, further studies regarding the methodology of use are required to obtain better seminal quality. This study aimed to compare two pharmacological semen collection times after the association of dexmedetomidine (30\u2009\u03bcg\/kg, IM; Dormitor\u00ae, Zoetis), ketamine (5\u2009mg\/kg, IM; ketamine, Vetnil) and urethral catheterization using a tomcat probe (0.8\u2009mm\u2009\u00d7\u20091.00\u2009mm\u2009\u00d7\u200911\u2009cm). The collections were divided into two experimental groups: G10 (<jats:italic>N<\/jats:italic>\u2009=\u20098; urethral catheterization after 10\u2009min of anaesthesia) and G15 (<jats:italic>N<\/jats:italic>\u2009=\u20098; urethral catheterization after 15\u2009min of anaesthesia). The ejaculates were evaluated for ejaculate volume, sperm concentration, morphology and kinetics using the CASA system. To compare the groups, the <jats:italic>t<\/jats:italic>\u2010test and the Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2010test were used with a significance level of 5%. It was identified that ejaculate volume (G10: 22.62\u2009\u00b1\u20092.13 vs. G15: 26.81\u2009\u00b1\u20091.55; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and sperm concentration (G10: 48.10\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009\u00b1\u200917.84 vs. G15: 90.18\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009\u00b1\u200919.35; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) was higher in G15 than in G10 and had a lower percentage of minor defects than G10 (G10: 3.12\u2009\u00b1\u20092.41 vs. G15: 1.00\u2009\u00b1\u20091.19; <jats:italic>p<\/jats:italic>\u2009=\u2009.043). Regarding the kinetic parameters, the results of G15 were better for total motility\u2014TM (G10: 67.00\u2009\u00b1\u200910.33 vs. G15: 81.87\u2009\u00b1\u20097.99; <jats:italic>p<\/jats:italic>\u2009=\u2009.006) and faster cells\u2014RAPID: (G10: 55.00\u2009\u00b1\u200916.63 vs. G15: 74.25\u2009\u00b1\u200911.94; <jats:italic>p<\/jats:italic>\u2009=\u2009.019); whereas a higher proportion of cells with slow speed\u2014SLOW were seen in G10 (G10: 31.00\u2009\u00b1\u200912.07 vs. 17.12\u2009\u00b1\u20097.53; <jats:italic>p<\/jats:italic>\u2009=\u2009.015). Based on these findings, we suggest that collection via urethral catheterization should be performed 15\u2009min after the application of ketamine\u2010associated dexmedetomidine to obtain a better\u2010quality ejaculate.<\/jats:p>","DOI":"10.1111\/rda.14420","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T08:11:42Z","timestamp":1688112702000},"page":"1207-1213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of two pharmacological semen collection times with \u03b12\u2010adrenergic agonist in domestic cats"],"prefix":"10.1111","volume":"58","author":[{"given":"Myrian Megumy Tsunokawa","family":"Hidalgo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Beatriz Marques","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia Amanda","family":"dos Santos\u00a0Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia Rodrigues","family":"Greghi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8623-5131","authenticated-orcid":false,"given":"Vin\u00edcius Wagner","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia Ribeiro","family":"Sambatti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Guilherme Corsi","family":"Trautwein","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8416-2450","authenticated-orcid":false,"given":"Maria Isabel Mello","family":"Martins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida \u2013 LARAA  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-70942002000500012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X15598550"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12855"},{"key":"e_1_2_10_5_1","unstructured":"Col\u00e9gio Brasileiro de Reprodu\u00e7\u00e3o Animal (CBRA). 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