{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:10:32Z","timestamp":1788311432562,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Synchronization of donor cells is an important step for the success of somatic cell nuclear transfer application and facilitates the development of embryos. Contact inhibition, serum starvation and different chemical agents are used in synchronizing different types of somatic cells. In this study, to synchronize the primary ovine adult (POF) and foetal (POFF) fibroblast cells to G0\/G1 phases, the contact inhibition, the serum starvation, roscovitine and trichostatin A (TSA) methods were used. In the first part of the study, roscovitine (10, 15, 20 and 30\u2009\u03bcM) and TSA (25, 50, 75 and 100\u2009nM) were applied for 24\u2009h to determine the optimal concentration for POF and POFF cells. In the second part, optimal concentrations of roscovitine and TSA for these cells were compared with contact inhibition and serum starvation methods. Cell cycle distribution and apoptotic activity analysis were performed by flow cytometry to compare this synchronization methods. Serum starvation method resulted in higher cell synchronization rate in both cells compared to other groups. Although contact inhibition and TSA also achieved high success rates of synchronized cell value, it was observed that the difference between serum starvation and these groups was significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). When the apoptosis rates of the two cell types were examined, it was observed that the early apoptotic cells in contact inhibition and late apoptotic cells in the serum starvation were higher than the other groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Although the 10 and 15\u2009\u03bcM concentrations of roscovitine gave the lowest apoptosis rates, it was observed that it failed to synchronize both the ovine fibroblast cells to G0\/G1 phase. As a result, it was concluded that while roscovitine was not successful to synchronize both the POFF and POF cell lines, TSA (50\u2009nM for POF cells and 100\u2009nM for POFF cells) can be used efficiently as an alternative to the contact inhibition and the serum starvation methods.<\/jats:p>","DOI":"10.1111\/rda.14425","type":"journal-article","created":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T20:25:55Z","timestamp":1688243155000},"page":"1251-1260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Can roscovitine and trichostatin A be alternatives to standard protocols for cell cycle synchronization of ovine adult and foetal fibroblast cells?"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9355-498X","authenticated-orcid":false,"given":"Selin","family":"Yagcioglu","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Istanbul University\u2010Cerrahpasa  Avc\u0131lar, Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7460-0450","authenticated-orcid":false,"given":"Nur","family":"Ersoy","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Institute of Graduate Studies Istanbul University\u2010Cerrahpasa  Avc\u0131lar, Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4561-6189","authenticated-orcid":false,"given":"Kamber","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Istanbul University\u2010Cerrahpasa  Avc\u0131lar, Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5069-5427","authenticated-orcid":false,"given":"Sema","family":"Birler","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Istanbul University\u2010Cerrahpasa  Avc\u0131lar, Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6200-3018","authenticated-orcid":false,"given":"Serhat","family":"Pabuccuoglu","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Istanbul University\u2010Cerrahpasa  Avc\u0131lar, Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.10\u2010058A"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2017.169"},{"key":"e_1_2_9_4_1","first-page":"1","article-title":"Production of cloned lambs: Transfer of early cleavage stage embryos to final recipients","volume":"36","author":"Birler S.","year":"2010","journal-title":"\u0130stanbul \u00dcniversitesi Veteriner Fak\u00fcltesi Dergisi"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod60.4.1013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/380064a0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(01)00699\u20109"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2005.00577.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.180324mc"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.09.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1501\/Vetfak_0000002885"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.017954"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.07.156"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.4.895"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2005.7.35"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102184.2011.00753.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192433399"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.18098"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.11.164"},{"issue":"3","key":"e_1_2_9_20_1","first-page":"297","article-title":"Improvement of mouse and sheep cloned blastocysts quality upon nuclear transfer of BRDT\u2014Expressing somatic cells","volume":"37","author":"Kotlarska M.","year":"2019","journal-title":"Animal Science Papers and Reports"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1139\/O09\u2010005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.11.007"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135344"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.06.018"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2012.1793"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201007\u20100397"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.03.016"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193954"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2009.01499.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.05.030"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10040545"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.07.027"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5346.2130"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199407004522"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.5.3637"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/385810a0"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3923\/javaa.2012.1812.1818"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2009.0038"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T06:01:56Z","timestamp":1694066516000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14425"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,10]]},"references-count":37,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/rda.14425"],"URL":"https:\/\/doi.org\/10.1111\/rda.14425","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,10]]},"assertion":[{"value":"2023-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}