{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:58:42Z","timestamp":1747187922177,"version":"3.40.5"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,8,6]],"date-time":"2023-08-06T00:00:00Z","timestamp":1691280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of assisted reproductive techniques, such as chilled semen, contributes to the maintenance and genetic improvement of canine breeding. The INRA\u201096 extender is a commercially available, chemically defined medium that was initially developed for the preservation of equine semen and exhibits preservation potential in the canine species. This research aims to evaluate the INRA\u201096 extender as an alternative for the short\u2010term preservation of canine semen in terms of sperm quality parameters such as motility and kinetic parameters, integrity and functionality of the plasma membrane in fresh and chilled\u2013rewarmed samples, as well as the sperm\u2010binding ability using the perivitelline membrane of the chicken egg as an indicator of the fertilizing capacity of the preserved semen. A total of 18 ejaculates from 9 French bulldogs (two ejaculates per dog) were collected and divided into two aliquots that were diluted in Tris\u2010egg yolk 20% (control) or INRA\u201096 to a final concentration of 100\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> sperm\/mL. Samples were refrigerated in a biological incubator at 5\u00b0C and evaluated at 0, 24 and 48\u2009h time points. Comparing the two treatments after 48\u2009h of refrigeration, both extenders showed similar values (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.5) for the majority of kinetic parameters, with the INRA\u201096 group promoting a total motility of 88.1\u2009\u00b1\u20092.9%. In addition, the morphology, integrity and functionality of the plasma membrane were preserved above 70% in this group. Dilution with INRA\u201096 also provided a significantly higher amount of sperm bound (256.2\u2009\u00b1\u200921.1) to the perivitelline membrane of the egg yolk compared to the sperm\u2010binding rates (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) achieved at the use of Tris\u2010egg yolk (215.2\u2009\u00b1\u200921 bound spermatozoa) at 48\u2009h. Our study proved similar functional properties of dog sperm cells treated with INRA\u201096 in comparison to commonly used home\u2010made Tris\u2010based extender during short\u2010time storage.<\/jats:p>","DOI":"10.1111\/rda.14448","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T04:51:39Z","timestamp":1691383899000},"page":"1320-1329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Short\u2010term preservation of canine sperm\u2010binding ability and other metrics using the <scp>INRA<\/scp>\u201096 in comparison to Tris\u2010egg yolk extender"],"prefix":"10.1111","volume":"58","author":[{"given":"Thales Pinheiro","family":"Cavalcanti","sequence":"first","affiliation":[{"name":"Universidade de Fortaleza  Fortaleza Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Gl\u00f3ria","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Conserva\u00e7\u00e3o de Germoplasma Animal Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana Grasiele Pereira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Conserva\u00e7\u00e3o de Germoplasma Animal Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samara Sandy Jer\u00f4nimo","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Conserva\u00e7\u00e3o de Germoplasma Animal Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreia Maria","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Conserva\u00e7\u00e3o de Germoplasma Animal Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri Gon\u00e7alves","family":"Matos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Conserva\u00e7\u00e3o de Germoplasma Animal Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5894-0148","authenticated-orcid":false,"given":"Pedro Nacib","family":"Jorge\u2010Neto","sequence":"additional","affiliation":[{"name":"Instituto Reprocon  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-1062","authenticated-orcid":false,"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Conserva\u00e7\u00e3o de Germoplasma Animal Universidade Federal Rural do Semi\u2010\u00c1rido  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.09.013"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2021.0054"},{"key":"e_1_2_10_4_1","unstructured":"Arruda R. P.(2000).Avalia\u00e7\u00e3o dos efeitos de diluidores e crioprotetores para o espermatozoide equino pelo uso de microscopia de epifluoresc\u00eancia citometria de fluxo an\u00e1lises computadorizadas da motilidade (CASA) e da morfometria (ASMA). [Evaluation of the effects of extenders and cryoprotectants on equine sperm using epifluorescence microscopy flow cytometry computerized motility analysis (CASA) and morphometry (ASMA)](Habilitation thesis). Universidade de S\u00e3o Paulo S\u00e3o Paulo p. 121."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40575\u2010021\u201000111\u20104"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13572"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20104320(01)00155\u20105"},{"key":"e_1_2_10_8_1","first-page":"252","article-title":"Refrigeration of canine sperm at +4\u00b0C: Comparative study of four different extenders for the refrigeration of canine sperm at +4\u00b0C: LDL, Tris egg yolk, Equex\u00ae, and INRA96\u00ae","volume":"164","author":"Bencharif D.","year":"2013","journal-title":"Revue de M\u00e9decine Veterinaire"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(98)00175\u20107"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.11606\/issn.1678\u20104456.bjvras.2017.127331"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.01.008"},{"key":"e_1_2_10_12_1","first-page":"146","article-title":"Different egg yolk concentrations on the quality of the canine semen diluted in ACP\u2010106 and chilled under 4\u00b0C","volume":"1","author":"Cardoso J. F. S.","year":"2010","journal-title":"Comunicata Scientiae"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.05.028"},{"key":"e_1_2_10_14_1","first-page":"104","volume-title":"Manual para exame androl\u00f3gico e avalia\u00e7\u00e3o de s\u00eamen animal [Manual for andrological examination and evaluation of animal semen]","author":"CBRA \u2013 Col\u00e9gio Brasileiro de Reprodu\u00e7\u00e3o Animal","year":"2013"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13596"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12070816"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.07.005"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.08.012"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0093\u2010691x(01)00440\u2010x"},{"key":"e_1_2_10_20_1","first-page":"287","volume-title":"Canine and feline theriogenology","author":"Johnston S. D.","year":"2001"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0195\u20105616(91)50054\u20101"},{"volume-title":"Recent advances in small animal reproduction","year":"2001","author":"Linde\u2010Forsberg C.","key":"e_1_2_10_22_1"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.11916"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2014.04.013"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.012"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2020.103104"},{"key":"e_1_2_10_27_1","first-page":"257","article-title":"Sperm morphology and fertility in the dog","volume":"47","author":"Oettl\u00e9 E. E.","year":"1993","journal-title":"Journal of Reproduction and Fertility \u2010 Supplement"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.2527\/1991.6983308x"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13349"},{"key":"e_1_2_10_30_1","first-page":"70","article-title":"Water\u2010induced hypo\u2010osmotic test for the evaluation of canine sperm membrane integrity","volume":"7","author":"Quintela A. T.","year":"2010","journal-title":"Animal Reproduction"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(02)01297\u20109"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100272.1992.tb02627.x"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(01)00609\u20104"},{"key":"e_1_2_10_34_1","first-page":"1586","article-title":"Collection and evaluation of canine semen: A review","volume":"5","author":"Robert M. A.","year":"2016","journal-title":"International Journal of Science, Environment and Technology"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20104320(00)00155\u2010x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.12.00"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.2002.0704"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201049203\u2010z"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2021\u2010111"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432\u20101327.1999.00203.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1590\/1984\u20103143\u2010AR2021\u20100069"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103\u20108478cr20210731"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T06:02:12Z","timestamp":1694066532000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,6]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/rda.14448"],"URL":"https:\/\/doi.org\/10.1111\/rda.14448","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2023,8,6]]},"assertion":[{"value":"2023-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}