{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:58:43Z","timestamp":1747187923179,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T00:00:00Z","timestamp":1691712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007923","name":"Yangtze University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In mammals, single blastomeres from as early as 2\u2010cell embryos demonstrate heterogeneous developmental capacity and fate decision into different cell lineages. However, mechanisms underlying blastomere heterogeneity of 2\u2010cell embryos remain largely unresolved. Here, we analysed the molecular heterogeneity of full\u2010length mRNAs and their 3\u2032UTR regions, based on the single\u2010cell RNA\u2010seq data of pig 2\u2010cell embryos generated from in vivo fertilization (in vivo), in vitro fertilization (in vitro) and parthenogenetic activation (PA), respectively. First, unsupervised clustering helped discover two different groups of blastomeres for 2\u2010cell pig embryos. Between these two groups of blastomeres in pig 2\u2010cell embryos, 35, 301 and 428 full\u2010length mRNAs respectively in in vivo, in vitro and PA embryo types were identified to be differentially expressed (<jats:italic>p<\/jats:italic><jats:sub>adj<\/jats:sub>\u2009\u2264\u2009.05 and |log<jats:sub>2<\/jats:sub>[fold change]|\u2009\u22651) (DE mRNAs), while 92, 89 and 42 mRNAs were shown to be with significantly different 3\u2032UTR lengths (3\u2032UTR DE) (<jats:italic>p<\/jats:italic><jats:sub>adj<\/jats:sub>\u2009\u2264\u2009.05). Gene enrichment for both DE mRNAs and 3\u2032UTR DE mRNAs found multiple signalling pathways, including cell cycle, RNA processing. Few numbers of common DE mRNAs and 3\u2032UTR DE mRNAs existed between in vitro and in vivo blastomeres derived from 2\u2010cell embryos, indicating the larger differences between in vitro and in vivo fertilized embryos. Integrative genomics viewer analysis further identified that 3\u2032UTRs of HSDL2 and SGTA (in vivo), FAM204A and phosphoserine phosphatase (in vitro), PRPF40A and RPIA (PA) had &gt;100\u2009nt average length changes. Moreover, numbers and locations of regulatory elements (polyadenylation site, cytoplasmic polyadenylation element and microRNA binding sites) within 3\u2032UTRs of these DE mRNAs were predicted. These results indicate that molecular heterogeneity existed among blastomeres from different types of pig 2\u2010cell embryos, providing useful information and novel insights into future functional investigation on its relationship with the subsequent embryo development and differentiation.<\/jats:p>","DOI":"10.1111\/rda.14454","type":"journal-article","created":{"date-parts":[[2023,8,12]],"date-time":"2023-08-12T04:19:57Z","timestamp":1691813997000},"page":"1393-1403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010cell <scp>RNA<\/scp> sequencing reveals blastomere heterogeneity of 2\u2010cell embryos in pigs"],"prefix":"10.1111","volume":"58","author":[{"given":"Ting","family":"Fang","sequence":"first","affiliation":[{"name":"College of Animal Science Yangtze University  Jingzhou 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