{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T05:28:46Z","timestamp":1768714126386,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T00:00:00Z","timestamp":1692403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Appropriate stallion semen handling is of great importance in equine artificial insemination (AI) industry. Optimal treatment of AI doses is aiming for best sperm preservation by excluding strong environmental influences and adding media that favour sperm survival. One method widely used in stallion sperm handling is the rotation of liquid\u2010preserved semen samples on roller benches during storage. As previous studies in boars give rise to the fact that rotation should not be considered beneficial for spermatozoa anymore, the present study investigated the influence of continuous rotation of diluted stallion AI portions on sperm quality. Ejaculates (<jats:italic>n<\/jats:italic>\u2009=\u200915) were collected at a German AI centre and diluted with the two extenders EquiPlus and Gent (Minit\u00fcb GmbH) to a final concentration of 50\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009sperm\/mL. Afterwards, samples were placed separately on roller benches at 5\u00b0C in the dark, obtaining a rotation frequency of 5 revolutions per minute (rpm) and 36\u2009rpm for four consecutive days following a split\u2010sample design. Both groups were analysed daily in comparison to a control group (0\u2009rpm) with an extended spectrum of spermatological methods including computer\u2010assisted sperm analysis and flow cytometry. Statistical analyses were based on the calculation of generalized linear mixed models for each sperm parameter. The research revealed a decrease in sperm quality parameters of rotated samples compared to non\u2010rotated control groups, visible in total sperm motility (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001), decreased thermo\u2010resistance (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and a drop in pH (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). Interestingly, no differences (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05) were detected between rotation frequencies of 5 and 36\u2009rpm. We conclude that the fertilizing capacity of stallion semen was negatively affected by rotation during storage in vitro, irrespective of the rotation frequency. Further studies need to investigate whether field fertility in horses is similarly affected by semen rotation on roller benches in vivo.<\/jats:p>","DOI":"10.1111\/rda.14456","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T07:39:14Z","timestamp":1692430754000},"page":"1413-1419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rotation of liquid\u2010preserved artificial insemination doses on roller benches affects sperm quality during storage in stallions"],"prefix":"10.1111","volume":"58","author":[{"given":"H.","family":"Dierberger","sequence":"first","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Pieper","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jung","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7932-9412","authenticated-orcid":false,"given":"M.","family":"Schulze","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now  Bernau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03089"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.06.025"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939\u20104640.2002.tb02623.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.014"},{"key":"e_1_2_10_6_1","volume-title":"Rotating flow","author":"Childs P. 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