{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:58:43Z","timestamp":1747187923028,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002753","name":"Universidad Nacional de Colombia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002753","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Low\u2010density lipoproteins (LDL), quercetin (Q) and resveratrol (R) have been used for sperm preservation to improve sperm quality in different species. To evaluate the effects of LDL, Q and R during the cooling of boar semen. Fifteen boar semen samples were diluted in a BTS extender supplemented with the treatments: LDL at 6%, Q at 10\u2009\u03bcM (Q10), 30\u2009\u03bcM (Q30) and 50\u2009\u03bcM (Q50), or R at 10\u2009\u03bcM (R10), 30\u2009\u03bcM (R30) and 50\u2009\u03bcM. A control without supplementation was included. The semen was stored by cooling at 16\u00b0C for 96\u2009h. Every 24\u2009h, sperm motility and kinetics were evaluated using a computer\u2010assisted sperm analyzer (IVOS). At 24 and 96\u2009h of cooling, functional membrane integrity and mitochondrial membrane potential (<jats:italic>\u0394\u03a8M<\/jats:italic>) of sperm were evaluated by the hypoosmotic swelling test (HOST) an flow cytometry with JC\u20101 probe, respectively, LDL improved progressive motility of sperm during cooling. Likewise, LDL increased average path velocity (VAP) and straight\u2010line velocity (VSL) and\/or curvilinear velocity (VCL) during the first 48\u2009h of cooling. The use of Q between 10 and 30\u2009\u03bcM caused a reduction in total motility, progressive motility and amplitude of the lateral head displacement during the entire cooling period, as well as a decrease in VAP, VSL and VCL at 96\u2009h of cooling. LDL, Q10, Q30 and Q50 modulated mitochondrial activity by reducing high\u2010<jats:italic>\u0394\u03a8M<\/jats:italic> sperm at 0 and 96\u2009h of cooling. During the cooling of the boar semen prior to artificial insemination, the parameters of sperm quality that could influence fertility decrease; however, the inclusion of antioxidants and additives that protect the plasma membrane, such as LDL, could mitigate the damaging effects on spermatozoa. It is concluded that LDL can improve the motility and kinetics of boar semen during cooling while it could modulating the sperm's mitochondrial activity. On the contrary, Q could alter the motility and kinetics of boar sperm during the cooling period.<\/jats:p>","DOI":"10.1111\/rda.14457","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T04:58:29Z","timestamp":1692248309000},"page":"1420-1427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low\u2010density lipoproteins, resveratrol and quercetin as alternative additives to improve boar semen cooling"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7333-6865","authenticated-orcid":false,"given":"Dar\u00edo","family":"Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Faculty of Sciences Universidad Nacional de Colombia  Medell\u00edn 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