{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:51:44Z","timestamp":1775029904252,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"publisher","award":["99001455"],"award-info":[{"award-number":["99001455"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004481","name":"University of Tehran","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004481","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bovine viral diarrhoea virus (BVDV) is an important viral agent causing the reproductive failure in cattle. The objectives of the study were to assess the role of male and female gametes, as carriers of cytopathic (CP) and non\u2010cytopathic (NCP) BVDV to embryonic cells during in vitro fertilization. In this respect, sperm and oocytes were separately exposed to concentrations of 10<jats:sup>4.5<\/jats:sup>or 10<jats:sup>5.5<\/jats:sup>TCID<jats:sub>50<\/jats:sub>\/mL CP and NCP BVDV, for 2\u2009h before fertilization. After washing, the intact gametes with the infected gametes were inseminated. Seven days post\u2010fertilization, the virus\u2010exposed embryos were examined for presence of the viral genome by RT\u2010PCR. One\u2010way<jats:sc>anova<\/jats:sc>with post\u2010hoc Tukey's HSD test and an independent samples<jats:italic>t<\/jats:italic>\u2010test were used to compare within and between groups, respectively. The results presented a significant decrease in the blastocyst rates for CP\u2010infected groups than NCP\u2010infected groups (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.01). Compared to the controls and the infected oocyte groups, the cleavage rates of the infected sperm groups (NCP and CP BVDV) were significantly reduced both in low (10<jats:sup>4.5<\/jats:sup>TCID<jats:sub>50<\/jats:sub>\/mL) and high (10<jats:sup>5.5<\/jats:sup>TCID<jats:sub>50<\/jats:sub>\/mL) titres of the virus (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.01). The proportion of embryos which was developed to blastocyst stages was significantly lower for CP and NCP\u2010infected groups than the control groups (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.001). According to the molecular results, all samples of the retarded\/degenerated embryos (at least one blastocyst within each one) in CP and NCP groups, one sample (at least one blastocyst in that) within a CP\u2010infected group, and six samples (at least one blastocyst in each one of those) of NCP\u2010infected groups contained the viral nucleic acid. Likewise, the results of viral enrichment showed all reactions in which RT\u2010PCR were positive induced CPEs in MDBK monolayers. In conclusion, it is clear that CP and NCP BVDV were able to traverse zona pellucida during fertilization, and they had also negative effects on embryo development.<\/jats:p>","DOI":"10.1111\/rda.14460","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T11:29:32Z","timestamp":1693308572000},"page":"1448-1455","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Sperm and oocyte as carriers for bovine viral diarrhoea virus biotypes during in vitro fertilization"],"prefix":"10.1111","volume":"58","author":[{"given":"Aidin","family":"Rahim\u2010Tayefeh","sequence":"first","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine University of Tehran Tehran Iran"},{"name":"Department of Animal Biotechnology National Institute of Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3673-2284","authenticated-orcid":false,"given":"Massoud","family":"Talebkhan\u2010Garoussi","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine University of Tehran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morteza","family":"Daliri\u2010Joupari","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology National Institute of Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1862-2233","authenticated-orcid":false,"given":"Farid","family":"Heidari","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology National Institute of Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Vahidi","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology National Institute of Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehran","family":"Bakhshesh","sequence":"additional","affiliation":[{"name":"Department of Animal Virology, Research and Diagnosis Razi Vaccine and Serum Research Institute (AREEO) Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3212-6137","authenticated-orcid":false,"given":"Abolfazl","family":"Shirazi","sequence":"additional","affiliation":[{"name":"Reproductive Biotechnology Research Center Avicenna Research Institute (ACECR) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.1992.tb00837.x"},{"issue":"6","key":"e_1_2_11_3_1","first-page":"1036","article-title":"Pseudorabies virus, porcine parvovirus, and porcine enterovirus interactions with the zona pellucida of the porcine embryo","volume":"44","author":"Bolin S. 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