{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T12:01:08Z","timestamp":1782907268507,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to evaluate the effect of supplementing bovine semen freezing extender with different concentrations of iodixanol on post\u2010thaw sperm characteristics. Six ejaculates of three Nellore bulls were pooled and diluted in commercial extender (BotuBov\u00ae) and then divided into 4 groups: control group (without adding iodixanol); groups G1.5, G3, or G6 according to the concentration of iodixanol solution (RedCushion\u00ae). After dilution, the samples were cooled and frozen. Post\u2010thaw semen evaluation included sperm motility by CASA immediately after thawing and after 60\u2009min of incubation at 37\u00b0C, flow cytometry analysis for integrity of plasma and acrosomal membranes, membrane destabilization and translocation of phosphatidylserine, mitochondrial membrane potential, and formation of intracellular anion superoxide (), hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>), and membrane lipid peroxidation. The group G6 presented significantly higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) total and progressive motility, percentage of plasma and acrosomal membrane integrity, and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>than control and group G1.5. Furthermore, group G6 showed lower (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) lipid peroxidation than control. In addition, regardless of the concentration used, the percentage of spermatozoa without phosphatidylserine translocation was higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) in all iodixanol supplemented groups. In conclusion, iodixanol supplementation preserved the motility and integrity of sperm membranes during cryopreservation and protected against lipid peroxidation.<\/jats:p>","DOI":"10.1111\/rda.14470","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T04:31:43Z","timestamp":1694147503000},"page":"1551-1558","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Iodixanol supplementation in freezing extender improves the antioxidant capacity of semen"],"prefix":"10.1111","volume":"58","author":[{"given":"Fernanda N.","family":"Marqui","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Alicio","family":"Martins","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine S\u00e3o Paulo State University (UNESP) Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tairini Erica","family":"da Cruz","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8536-3956","authenticated-orcid":false,"given":"Tatiana Issa Uherara","family":"Berton","sequence":"additional","affiliation":[{"name":"Tairana Artificial Insemination Station S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"de Paula\u00a0Freitas\u2010Dell\u2019Aqua","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Jos\u00e9 A.","family":"Dell\u2019Aqua","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0333-7437","authenticated-orcid":false,"given":"Eunice","family":"Oba","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science S\u00e3o Paulo State University (UNESP) Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2019.20.1.79"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.01.011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.10.031"},{"key":"e_1_2_9_5_1","first-page":"1","article-title":"Involvement of bicarbonate\u2010induced radical signaling in oxysterol formation and sterol depletion of capacitating mammalian sperm during in vitro fertilization","volume":"88","author":"Boerke A.","year":"2014","journal-title":"Biology of Reproduction"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1340707"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2010.09.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.02.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12798"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.03.010"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5433\/1679-0359.2015v36n6Supl2p4365"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp0618680"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5216\/cab.v12i3.9739","article-title":"Avalia\u00e7\u00e3o Da Integridade De Membrana Em Espermatozoide Bovino Criopreservado Para Prever O \u00cdndice De Prenhez","volume":"12","author":"Emerick L. 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