{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T03:35:53Z","timestamp":1774064153813,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mammalian spermatogenesis is a highly complicated and intricately organized process involving spermatogonia propagation (mitosis) and meiotic differentiation into mature sperm cells (spermiogenesis). In pigs, spermatogonia development and the role of somatic cells in spermatogenesis were previously investigated in detail. However, the characterization of key molecules fundamental to pig spermiogenesis remains less explored. Here we compared spermatogenesis between humans and pigs, focusing on spermiogenesis, by integrative testicular single\u2010cell RNA sequencing (scRNA\u2010seq) analysis. Human and pig testicular cells were clustered into 26 different groups, with cell\u2010type\u2010specific markers and signalling pathways. For spermiogenesis, pseudo\u2010time analysis classified the lineage differentiation routes for round, elongated spermatids and spermatozoa. Moreover, markers and molecular pathways specific to each type of spermatids were examined for humans and pigs, respectively. Furthermore, high\u2010dimensional weighted gene co\u2010expression network analysis (hdWGCNA) identified gene modules specific for each type of human and pig spermatids. Hub genes (pig: SNRPD2.1 related to alternative splicing; human: CATSPERZ, Ca[2+] ion channel) potentially involved in spermiogenesis were also revealed. Taken together, our integrative analysis found that human and pig spermiogeneses involve specific genes and molecular pathways and provided resources and insights for further functional investigation on spermatid maturation and male reproductive ability.<\/jats:p>","DOI":"10.1111\/rda.14493","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T17:40:30Z","timestamp":1698169230000},"page":"1745-1755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integrated single cell transcriptome sequencing analysis reveals species\u2010specific genes and molecular pathways for pig spermiogenesis"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8354-4479","authenticated-orcid":false,"given":"Ya\u2010Dan","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Animal Science Yangtze University  Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-8281","authenticated-orcid":false,"given":"Cai\u2010Xia","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science Yangtze University  Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Qiang","family":"Du","sequence":"additional","affiliation":[{"name":"College of Animal Science Yangtze University  Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2010.0024"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.01.035"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2010.01100.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202300756R"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.146571"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.82191"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0439-x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-014-1216-z"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1605"},{"issue":"4","key":"e_1_2_10_11_1","first-page":"1026","article-title":"G protein\u2010coupled estrogen receptor promotes acrosome reaction via regulation of Ca2+ signaling in mouse sperm","volume":"107","author":"Gao D. D.","year":"2022","journal-title":"Biology of Reproduction"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.07.025"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007810"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106896"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-018-0099-2"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.12.005"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1114870"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.10.026"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkac130"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105733"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00028.2022"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043127"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.05.015"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.07.018"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12810"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-021-00894-z"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05547-7"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.04.009"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.24.110707.175355"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4161\/21565562.2014.979623"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes14020383"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-080320-040045"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex270"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.944325"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24140-6"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-98267-3"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3192"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.05.010"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1593"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.045"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34990-3"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731118000435"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12502"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38199-w"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202200152R"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1119514"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaad004"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deac157"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.08.007"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac216"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24097982"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac109"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.11956"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2023.2183339"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001035RRR"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2022.03.014"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-021-00638-3"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2022.037"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38357-0"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-022-00687-2"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14493","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T06:23:55Z","timestamp":1703312635000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,24]]},"references-count":61,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/rda.14493"],"URL":"https:\/\/doi.org\/10.1111\/rda.14493","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,24]]},"assertion":[{"value":"2023-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}