{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T08:31:35Z","timestamp":1769761895822,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T00:00:00Z","timestamp":1698883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Milk production traits as the most important economic traits of dairy cows, they directly reflect the benefits of breeding and the economic benefits of pasture. In this study, A disintegrin and metalloproteinase\u201012 (<jats:italic>ADAM12<\/jats:italic>), Parkinson's disease gene 2 (<jats:italic>PRKN<\/jats:italic>) and dipeptidyl peptidase\u2010like protein subtype 6 (<jats:italic>DPP6<\/jats:italic>) polymorphism in 384 Chinese Holstein cows were detected by time\u2010of\u2010flight mass spectrometry and through statistical analysis using software such as Popgene 32, SAS 9.4 and Origin 2022, the relationship between single nucleotide polymorphisms (SNPs) of three genes with four milk production traits such as daily milk yield (DMY), milk fat percentage (MFP), milk protein percentage (MPP) and somatic cell score (SCS) was verified at molecular level. The results showed that four polymorphic loci (116,467,133, 116,604,487, 116,618,268 and 116,835,111) of <jats:italic>DPP6<\/jats:italic> gene, two polymorphic loci (97,665,052 and 97,159,837) of <jats:italic>PRKN<\/jats:italic> gene and two polymorphic loci (45,542,714 and 45,553,888) of <jats:italic>ADAM12<\/jats:italic> gene were detected. <jats:italic>PRKN<\/jats:italic>\u201097665052, <jats:italic>DPP6<\/jats:italic>\u2010116467133, <jats:italic>ADAM12<\/jats:italic>\u201045553888, <jats:italic>DPP6<\/jats:italic>\u2010116604487 and <jats:italic>DPP6<\/jats:italic>\u2010116835111 were all in Hardy\u2013Weinberg equilibrium state (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). <jats:italic>ADAM12<\/jats:italic>\u201045542714, <jats:italic>PRKN\u2010<\/jats:italic>97159837 and <jats:italic>PRKN\u2010<\/jats:italic>97665052 were moderately polymorphic (0.25\u2009\u2264\u2009<jats:italic>PIC<\/jats:italic>\u2009&lt;0.50) in Holstein. It is evident that the selection potential and genetic variation of these five loci are relatively large, and the genetic richness is relatively high. The correlation analysis of different genotypes between these eight loci and milk production traits of Holstein showed that <jats:italic>ADAM12<\/jats:italic>\u201045542714 and <jats:italic>DPP6<\/jats:italic>\u2010116835111 (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) had an extremely significant effects on the DMY of Chinese Holstein in Ningxia, while <jats:italic>PRKN<\/jats:italic>\u201097665052 had an extremely significant effect on MFP (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). The effect of <jats:italic>PRKN<\/jats:italic>\u201097665052 and <jats:italic>DPP6<\/jats:italic>\u2010116467133 on MPP of Holstein were extremely significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). <jats:italic>DPP6\u2010<\/jats:italic>116618268 had an extremely significant effect on the SCS of Holstein in Ningxia (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), and <jats:italic>AA<\/jats:italic> genotype individuals showed a higher SCS than <jats:italic>GG<\/jats:italic> genotype individuals; the other two loci (<jats:italic>ADAM12<\/jats:italic>\u201045553888 and <jats:italic>DPP6\u2010<\/jats:italic>116604487) had no significant effects on milk production traits of Holstein (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). In addition, through the joint analysis of <jats:italic>DPP6<\/jats:italic>, <jats:italic>PRKN<\/jats:italic> and <jats:italic>ADAM12<\/jats:italic> gene loci, it was found that the interaction effect between the three gene loci could significantly affect the DMY, SCS (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and MPP (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). In conclusion, several different loci of <jats:italic>DPP6<\/jats:italic>, <jats:italic>PRKN<\/jats:italic> and <jats:italic>ADAM12<\/jats:italic> genes can affect the milk production traits of Holstein to different degrees. <jats:italic>PRKN<\/jats:italic>, <jats:italic>DPP6<\/jats:italic> and <jats:italic>ADAM12<\/jats:italic> genes can be used as potential candidate genes for milk production traits of Holstein for marker\u2010assisted selection, providing theoretical basis for breeding of Holstein.<\/jats:p>","DOI":"10.1111\/rda.14497","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T16:58:37Z","timestamp":1698944317000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Polymorphism of <i>ADAM12<\/i>, <i>DPP6<\/i> and <i>PRKN<\/i> genes and their associations with milk production traits in Holstein"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7213-1791","authenticated-orcid":false,"given":"Rui\u2010Rui","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010hong","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010li","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010fen","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei","family":"Cai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Yun","family":"Han","sequence":"additional","affiliation":[{"name":"Ningxia Agriculture Reclamation Helanshan dairy Co.Ltd.  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4881-0761","authenticated-orcid":false,"given":"Yun","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology Ningxia University  Yinchuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10709\u2010008\u20109312\u20104"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2018.1523537"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/tas\/txz083"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711\u2010020\u201000574\u20102"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c07763"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00790"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1720427"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/age.13192"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/139.2.907"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.129"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1150"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31109"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010021\u201001002\u20100"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010018\u20100082\u2010y"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201540352"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.15.0476"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.578070"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00084"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12192542"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2017.12.013"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20170476"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711\u2010016\u20100190\u20104"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12111830"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2022.2039169"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201085094\u20109"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002172200"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911\u2010008\u20109084\u20106"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489847"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.924"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13081437"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14497","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T06:31:47Z","timestamp":1706164307000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,2]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/rda.14497"],"URL":"https:\/\/doi.org\/10.1111\/rda.14497","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,2]]},"assertion":[{"value":"2023-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14497"}}